Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EO946_RS12490 Genome accession   NZ_CP034943
Coordinates   2433197..2434267 (-) Length   356 a.a.
NCBI ID   WP_003226312.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2428197..2439267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS12445 (EO946_12445) tapA 2428678..2429439 (-) 762 WP_003226335.1 amyloid fiber anchoring/assembly protein TapA -
  EO946_RS12450 (EO946_12450) - 2429708..2430034 (+) 327 WP_079996407.1 YqzG/YhdC family protein -
  EO946_RS12455 (EO946_12455) - 2430076..2430255 (-) 180 WP_003226330.1 YqzE family protein -
  EO946_RS12460 (EO946_12460) comGG 2430327..2430701 (-) 375 WP_003226328.1 competence type IV pilus minor pilin ComGG Machinery gene
  EO946_RS12465 (EO946_12465) comGF 2430702..2431085 (-) 384 WP_032727308.1 competence type IV pilus minor pilin ComGF Machinery gene
  EO946_RS12470 (EO946_12470) comGE 2431111..2431458 (-) 348 WP_003226323.1 competence type IV pilus minor pilin ComGE Machinery gene
  EO946_RS12475 (EO946_12475) comGD 2431442..2431873 (-) 432 WP_003226321.1 competence type IV pilus minor pilin ComGD Machinery gene
  EO946_RS12480 (EO946_12480) comGC 2431863..2432159 (-) 297 WP_003226319.1 comG operon protein ComGC Machinery gene
  EO946_RS12485 (EO946_12485) comGB 2432173..2433210 (-) 1038 WP_003226315.1 competence type IV pilus assembly protein ComGB Machinery gene
  EO946_RS12490 (EO946_12490) comGA 2433197..2434267 (-) 1071 WP_003226312.1 competence protein ComGA Machinery gene
  EO946_RS12500 (EO946_12500) - 2434480..2434890 (-) 411 WP_032727412.1 CBS domain-containing protein -
  EO946_RS12505 (EO946_12505) - 2434953..2435906 (-) 954 WP_003226308.1 magnesium transporter CorA family protein -
  EO946_RS12510 (EO946_12510) - 2436050..2437378 (+) 1329 WP_044428668.1 hemolysin family protein -
  EO946_RS12515 (EO946_12515) - 2437430..2438269 (-) 840 WP_003226304.1 STAS domain-containing protein -
  EO946_RS12525 (EO946_12525) mgsR 2438495..2438872 (-) 378 WP_003226302.1 transcriptional regulator MgsR -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40489.75 Da        Isoelectric Point: 9.0214

>NTDB_id=333591 EO946_RS12490 WP_003226312.1 2433197..2434267(-) (comGA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MDSIEKISKTLIEEAYITKASDIHIVPRERDAIIHFRVDHALLKKRHMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLEEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAKIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQLRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRHIIRKGIALGYLTTNNYDRWVYHETD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=333591 EO946_RS12490 WP_003226312.1 2433197..2434267(-) (comGA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
TTGGATTCAATAGAAAAGATAAGCAAAACCTTGATTGAAGAGGCATATATAACAAAGGCTTCTGACATTCACATTGTACC
GAGGGAGCGGGACGCCATCATTCATTTTCGGGTTGATCATGCCTTGCTGAAAAAAAGGCACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTCTCAGCGATGGACATAGGGGAAAGGAGGAAGCCGCAAAATGGTTCGCTTACA
TTAAAGCTAGAGGAGGGAAATGTTCATTTAAGAATGTCAACGCTCCCCACAATTAATGAAGAAAGCCTTGTGATCAGAGT
GATGCCTCAATATAATATCCCTTCGATTGATAAGCTGTCATTATTTCCGAAGACAGGAGCCACTTTACTCTCATTTTTAA
AACATTCCCACGGCATGCTCATTTTTACCGGGCCGACTGGTTCAGGGAAGACCACCACATTATACTCTCTTGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACACTGGAGGACCCTGTTGAAACAAGGGACGAGGATGTTCTTCAGGTGCA
GGTGAATGAAAAAGCCGGTGTGACATATTCCGCAGGGCTGAAGGCTATTTTGCGCCATGACCCGGATATGATTATCTTAG
GTGAGATCAGAGACGCCGAAACAGCTAAAATCGCGGTACGGGCAGCGATGACGGGGCATCTGGTGCTGACGAGCCTTCAT
ACGAGAGATGCGAAGGGCGCGATTTACAGGCTGCTTGAATTCGGCATCAATATGAATGAAATCGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGCCAGTTAC
GAAACACCAGGAGAGCAAGCGTTTATGAGCTCCTTTACGGAAAAAATCTTCAGCAATGTATTCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTACGTCACATTATCCGAAAAGGAATTGCACTCGGCTATTTGACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAACAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

97.472

100

0.975

Multiple sequence alignment