Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   EO946_RS05740 Genome accession   NZ_CP034943
Coordinates   1113393..1117097 (+) Length   1234 a.a.
NCBI ID   WP_003224421.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1108393..1122097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS05725 (EO946_05725) - 1108839..1109168 (-) 330 WP_032727222.1 four-helix bundle copper-binding protein -
  EO946_RS05730 (EO946_05730) - 1109285..1109722 (-) 438 WP_003224414.1 ferritin family protein -
  EO946_RS05735 (EO946_05735) addB 1109906..1113406 (+) 3501 WP_003224417.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  EO946_RS05740 (EO946_05740) addA 1113393..1117097 (+) 3705 WP_003224421.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  EO946_RS05745 (EO946_05745) sbcD 1117169..1118344 (+) 1176 WP_003224424.1 exonuclease subunit SbcD -
  EO946_RS05750 (EO946_05750) sbcC 1118341..1121733 (+) 3393 WP_003224426.1 exonuclease subunit SbcC -
  EO946_RS05755 (EO946_05755) hlpB 1121747..1122049 (+) 303 WP_003224428.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141455.81 Da        Isoelectric Point: 5.0516

>NTDB_id=333563 EO946_RS05740 WP_003224421.1 1113393..1117097(+) (addA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEENPIDVDQLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGEKA
FFELVDRYTTDRHDLDLQFLVKQVYEYSRSHPNPEAWLESFVHLYDVSEKSAIEELPFYQYVKEDIEMVLNGAKEKLQRA
LELTKVPGGPAPRADNFLDDLAQIDELIQHQDDFTELYKRVPAVSFKRVKAVKGDEFDPTLLEEATDLRNGAKKLLEKLK
TDYFTRSPEQHLKSLADMKPVIETLVRLVISYGKRFEAAKQEKSIIDFSDLEHYCLSILTAENETGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGTGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPDNDETETELLLIDNAEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFEAVEVAVALSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLETFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFAAGLGRNFNMMDLNKSYLLDKELGFGTKYIHPQLRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
IGSCKDHQKQLAKWQASASQTDWLLPEFDRYQAKTYLDFIGPALARHRDMEVLAENGAATHADISGHPARFAVRMIHSYD
LLDDDLEERTEEKSERLEAIRKGEPVPGTFAFDEKAREQLSWTYPHHEVTQIRTKQSVSEIKRKREYEDEYSGRAPVKPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHIPTIEEAEQTVHKLYEKELLTEEQKTAIDIEEIVQFFHTEIGG
QLIGAKWKDREVPFSLALPAKEIYPDAQEADEPLLVQGIIDCLYETEDGLYLLDYKSDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTKAVEQIAKTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=333563 EO946_RS05740 WP_003224421.1 1113393..1117097(+) (addA) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAATGGAATGCCATTGTCTCAACCGGCCAGGATATTCT
CGTAGCAGCGGCGGCGGGCTCCGGTAAAACCGCTGTGCTCGTTGAACGAATGATCCGGAAAATCACCGCGGAAGAAAACC
CGATTGATGTAGACCAGCTTCTTGTTGTGACATTTACAAACGCCTCAGCGGCAGAGATGAAACACCGGATCGCGGATGCC
CTGGAAAAAGAGCTTGTAAAACGCCCCGGCTCGCTGCATATCAGGCGCCAGCTGTCTCTTTTAAACCGGGCCAGCATTTC
GACACTTCATTCCTTTTGCCTGCAAGTGCTGAAGAAATATTACTACTTGATCGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAGCTGCTCGGGGATGAGGTGCTGGACGAGCTGTTTGAAGACGAATATGCAAAGGGCGAAAAGGCG
TTTTTTGAGCTTGTTGACCGCTACACGACAGATCGGCACGATCTGGATCTGCAATTTCTCGTCAAACAGGTGTACGAGTA
TTCCCGTTCCCACCCAAACCCGGAGGCATGGCTGGAAAGCTTTGTTCATTTGTATGATGTATCGGAAAAGAGTGCCATCG
AGGAGCTTCCGTTTTATCAATATGTCAAAGAGGATATTGAGATGGTGCTTAACGGAGCGAAGGAAAAGCTTCAGCGCGCG
CTTGAACTGACGAAAGTGCCGGGCGGTCCCGCGCCGCGTGCTGACAATTTTCTTGATGATCTTGCGCAGATTGATGAACT
GATTCAGCATCAGGATGATTTTACTGAGCTTTATAAGCGGGTGCCCGCAGTCTCTTTTAAGCGCGTGAAAGCAGTAAAAG
GGGACGAGTTTGATCCTACACTCCTTGAGGAAGCGACAGATTTGAGAAACGGTGCAAAAAAACTGCTTGAAAAGCTTAAA
ACCGACTACTTCACGAGAAGTCCTGAACAGCATTTAAAAAGCCTAGCCGACATGAAGCCTGTTATTGAAACGCTCGTACG
GCTTGTCATCAGCTATGGAAAACGGTTCGAAGCCGCGAAGCAGGAAAAATCAATCATTGATTTTTCTGATTTGGAGCATT
ACTGTTTATCCATTTTGACAGCTGAGAATGAAACAGGTGAACGCGAGCCGAGCGAGGCAGCAAGATTTTATCAAGAGCAG
TTTCATGAGGTGCTCGTTGACGAATATCAGGATACCAACCTAGTGCAGGAATCGATCCTGCAGCTCGTCACAAGCGGACC
TGAGGAAACAGGCAACCTGTTTATGGTAGGAGATGTCAAACAGTCAATTTACCGATTCAGGCTTGCGGAGCCGCTTCTAT
TCCTCTCCAAATACAAACGGTTTACAGAAAGTGGAGAAGGCACGGGACGGAAAATTGACTTGAATAAGAATTTCCGAAGC
CGGGCTGATATTTTAGATAGCACAAACTTTCTATTTAAACAACTGATGGGCGGCAAAATCGGTGAGGTTGATTATGACGA
GCAGGCTGAGCTGAAGCTTGGTGCGGCGTATCCGGACAATGATGAGACGGAAACGGAGCTGCTGCTGATCGACAACGCAG
AAGAGACGGATGCAGGCGAAGAAGCAGAAGAGCTGGAAACGGTGCAGTTTGAGGCGAAAGCGATCGCTAAGGAAATTCGC
AAGCTGATTTCATCGCCATTTAAGGTGTATGACGGAAAAACGAAAACACACCGCAATATTCAATACCGCGATATTGTGAT
TTTGCTCCGCTCCATGCCGTGGGCACCGCAAATCATGGAGGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CGTCAGGCTATTTTGAAGCGGTCGAAGTTGCCGTCGCGCTTTCTGTGCTGAAGGTGATCGATAACCCGTATCAGGATATA
CCGCTTGCCTCTGTTCTACGCTCACCGATTGTCGGAGCAGATGAAAACGAGCTGTCTTTGATCCGGCTTGAAAATAAAAA
AGCGCCGTACTATGAAGCGATGAAAGACTATTTGGCTGCCGGCGACCGGAACGACGAGCTTTATCAAAAGCTGGAAACGT
TCTACGGGCATCTTCAAAAATGGCGTGCATTTTCGAAAAACCACTCAGTATCTGAGCTGATTTGGGAAGTGTACCGCGAC
ACGAAATACATGGATTATGTCGGCGGAATGCCGGGCGGAAAACAGCGCCAGGCCAATTTGCGAGTTCTCTATGACCGGGC
GCGCCAATATGAATCGACGGCATTTCGCGGCCTGTTCCGTTTCCTGCGATTTATCGAACGCATGCAGGAGCGGGGCGATG
ATCTTGGAACGGCGCGAGCGCTCAGCGAGCAGGAGGATGTCGTCCGCTTAATGACGATCCACAGCAGCAAAGGGCTCGAA
TTTCCCGTCGTGTTTGCAGCAGGTCTCGGCCGGAATTTTAACATGATGGACTTGAACAAATCGTACCTGCTGGACAAGGA
GCTTGGGTTCGGCACAAAGTATATTCATCCGCAATTGCGCATCAGCTACCCGACGCTGCCGCTCATTGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCAGAGGAATTGCGTGTTCTCTATGTTGCATTAACGAGAGCGAAAGAAAAACTGTTTCTG
ATTGGCTCGTGCAAGGATCATCAAAAACAGCTCGCAAAATGGCAGGCATCCGCGTCCCAGACTGACTGGCTTCTTCCGGA
ATTTGACCGTTATCAGGCAAAAACGTATTTAGATTTCATTGGACCGGCTCTTGCGAGACACAGAGATATGGAGGTTTTGG
CTGAAAACGGTGCGGCAACGCATGCTGATATATCAGGACACCCGGCTCGTTTTGCCGTTCGAATGATTCATTCCTATGAT
TTACTGGATGATGATCTGGAAGAAAGGACGGAAGAAAAAAGCGAGCGCTTAGAAGCGATCCGCAAAGGTGAACCAGTTCC
CGGCACGTTTGCATTTGATGAAAAAGCCCGCGAGCAGTTGAGCTGGACATACCCGCATCACGAGGTCACGCAAATTCGGA
CAAAGCAGTCGGTTTCTGAGATCAAGAGAAAAAGAGAGTACGAGGATGAATACAGCGGCCGGGCCCCCGTTAAACCTGCT
GACGGAAGCATTCTGTACAGGCGCCCCGCTTTCATGATGAAAAAAGGCCTGACAGCGGCGGAGAAAGGGACGGCCATGCA
TACGGTTATGCAGCATATCCCGCTTACACATATCCCAACGATAGAAGAAGCGGAGCAGACGGTTCACAAGCTTTATGAAA
AAGAACTGCTCACTGAAGAACAAAAAACCGCTATTGATATAGAAGAAATCGTGCAATTTTTCCATACAGAAATCGGCGGA
CAGCTGATCGGCGCTAAATGGAAGGACCGGGAAGTGCCATTTAGTTTAGCGCTTCCGGCCAAGGAAATCTATCCTGATGC
ACAGGAGGCAGATGAACCGCTTTTAGTGCAGGGGATCATCGACTGTCTCTATGAAACTGAGGACGGCTTATATCTCTTGG
ACTATAAATCAGACCGGATTGAGGGCAAGTTCCAACACGGCTTTGAAGGAGCGGCCCCGATCTTGAAGAAACGATATGAA
ACGCAAATTCAGCTGTACACGAAGGCAGTTGAGCAAATTGCAAAAACAAAGGTAAAGGGATGTGCGCTGTATTTCTTTGA
CGGAGGGCACATTCTGACATTATAG

Domains

Predicted by InterproScan.

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-882)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

96.515

100

0.965

Multiple sequence alignment