Detailed information NTG0332546

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ELR50_RS15395
• Genome accession: NZ_CP034688
• Coordinates: 3436272..3437417 (-)
• Length: 381 a.a.
• NCBI ID: WP_009615238.1
• Uniprot ID: A0A1A5D0E6
• Organism: Pseudomonas citronellolis strain WXP-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3431272..3442417

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ELR50_RS15380 (ELR50_15300)	-	3432725..3433897 (-)	1173	WP_043269495.1	cystathionine gamma-synthase	-
ELR50_RS15385 (ELR50_15305)	-	3433894..3435267 (-)	1374	WP_043269497.1	pyridoxal-phosphate dependent enzyme	-
ELR50_RS15390 (ELR50_15310)	-	3435574..3435978 (-)	405	WP_043269499.1	NINE protein	-
ELR50_RS15395 (ELR50_15315)	pilU	3436272..3437417 (-)	1146	WP_009615238.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ELR50_RS15400 (ELR50_15320)	pilT	3437522..3438556 (-)	1035	WP_009615237.1	type IV pilus twitching motility protein PilT	Machinery gene
ELR50_RS15405 (ELR50_15325)	-	3438630..3439319 (+)	690	WP_249918055.1	YggS family pyridoxal phosphate-dependent enzyme	-
ELR50_RS15410 (ELR50_15330)	proC	3439336..3440154 (+)	819	WP_269961755.1	pyrroline-5-carboxylate reductase	-
ELR50_RS15415 (ELR50_15335)	-	3440165..3440758 (+)	594	WP_009615234.1	YggT family protein	-
ELR50_RS15420 (ELR50_15340)	-	3440898..3441194 (+)	297	WP_043269503.1	DUF167 domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 42685.06 Da
• Isoelectric Point: 6.7792

>NTDB_id=332546 ELR50_RS15395 WP_009615238.1 3436272..3437417(-) (pilU) [Pseudomonas citronellolis strain WXP-4]
MEFEKLLRLMVEKGASDLFVTAGVPPSMKVNGKVLPVTKTPLSPEQTRETVLAVMNEQQRRDFAEHHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTIEDLKLPEILKKLAMTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDTFEVALKNTLRQAPDVIMIGEVRSRETMEHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHQQIWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYSQGEITYEDALAYADSANDLRLMIKLGSETDADHLSSMTQGLTLELSEDDPNRRFR

Nucleotide

• Length: 1146 bp

>NTDB_id=332546 ELR50_RS15395 WP_009615238.1 3436272..3437417(-) (pilU) [Pseudomonas citronellolis strain WXP-4]
ATGGAATTCGAGAAGCTTCTGCGGCTGATGGTGGAGAAGGGCGCCTCGGACCTCTTCGTCACCGCCGGCGTGCCCCCGTC
GATGAAGGTCAACGGCAAGGTGCTGCCGGTGACCAAGACCCCGCTGTCGCCCGAGCAGACCCGCGAGACGGTGCTGGCGG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGCACCACGAGTGCAACTTCGCCATCAGCGCCCGGGGCATCGGGCGT
TTCCGCGTCAGCGCCTTCTACCAGCGCAACCTGGTGGGCATGGTGTTGCGCCGCATCGAGACCAACATCCCGACCATCGA
GGACCTCAAGCTGCCGGAGATTCTCAAGAAGCTGGCCATGACCAAGCGCGGGCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACTTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAATTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACACCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGCGAGACCATGGAGCACG
CAGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGGCACCAGCAGATCTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCTGACGGCAAGGGCCGCCGCGCGGTGATCGAGGTGCTGCTGAATACCCCGTTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTATCAGCTCTACTCCCAGGGCGAGATCACCTATGAGGATGCGCTGGCCTATGCCGACTCGGCCAACGACCTGCGGCT
GATGATCAAGCTCGGGTCGGAGACCGATGCGGATCATCTGTCGAGCATGACTCAGGGGTTGACCCTGGAGCTGAGCGAAG
ACGATCCGAATCGCCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-270)

3D structure

• AlphaFold DB: A0A1A5D0E6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	87.664	100	0.877
pilU	Acinetobacter baylyi ADP1	63.032	98.688	0.622
pilU	Vibrio cholerae strain A1552	60.571	91.864	0.556
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.363	88.976	0.386