Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ELR50_RS05525 Genome accession   NZ_CP034688
Coordinates   1290841..1292043 (-) Length   400 a.a.
NCBI ID   WP_176707187.1    Uniprot ID   -
Organism   Pseudomonas citronellolis strain WXP-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1285841..1297043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELR50_RS05500 (ELR50_05460) - 1286032..1286865 (+) 834 WP_269963422.1 oxidoreductase -
  ELR50_RS05505 (ELR50_05465) - 1287376..1287648 (-) 273 WP_269963423.1 hypothetical protein -
  ELR50_RS05510 (ELR50_05470) - 1288111..1289052 (-) 942 WP_269963424.1 MBL fold metallo-hydrolase -
  ELR50_RS29850 (ELR50_05475) - 1289580..1289846 (+) 267 WP_416236130.1 tlde1 domain-containing protein -
  ELR50_RS05515 (ELR50_05480) - 1289843..1290181 (+) 339 WP_043266595.1 hypothetical protein -
  ELR50_RS05520 (ELR50_05485) - 1290171..1290398 (-) 228 Protein_1100 hypothetical protein -
  ELR50_RS05525 (ELR50_05490) pilU 1290841..1292043 (-) 1203 WP_176707187.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ELR50_RS05530 (ELR50_05495) - 1292235..1292513 (+) 279 WP_009619516.1 peptidylprolyl isomerase -
  ELR50_RS05535 (ELR50_05500) - 1292527..1292889 (+) 363 WP_269963425.1 MmcQ/YjbR family DNA-binding protein -
  ELR50_RS05540 (ELR50_05505) - 1292886..1293443 (+) 558 WP_269963426.1 putative glycolipid-binding domain-containing protein -
  ELR50_RS05545 (ELR50_05510) - 1293713..1294912 (+) 1200 WP_269963427.1 MFS transporter -
  ELR50_RS05550 (ELR50_05515) - 1295739..1297019 (+) 1281 WP_069866035.1 OprD family porin -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 43903.04 Da        Isoelectric Point: 6.0779

>NTDB_id=332530 ELR50_RS05525 WP_176707187.1 1290841..1292043(-) (pilU) [Pseudomonas citronellolis strain WXP-4]
MEIQAMLRILSSQDGSDLYLSTGAPPCAKFNGVLKPLSSEPLKAGDVARIADAIMDEEQKAEFERELEMNLAISVAGVGR
FRINIFKQRNEVSIVARNIKLDIPLFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAISEEEAVKNADSANNLRLKLKLHRDNPAAAQTQAASAQPATPAAPAVEASSWGLELALEEIKAEDEEDKKGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=332530 ELR50_RS05525 WP_176707187.1 1290841..1292043(-) (pilU) [Pseudomonas citronellolis strain WXP-4]
ATGGAAATCCAAGCGATGCTGCGGATCCTGTCCAGCCAGGACGGTTCCGACCTTTACCTTTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGTCCAGCGAACCGCTGAAGGCCGGCGACGTGGCGCGCATCGCCGACGCCA
TCATGGACGAGGAACAGAAGGCCGAATTCGAGCGCGAGCTGGAGATGAACCTGGCGATCTCGGTCGCCGGCGTCGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCTGTTCGA
AGACCTGAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGGCACATCATTACCATCGAGGAC
CCCATCGAGTACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGCGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGGCACCTGGCGATCTCCACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAACTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCACAGCG
CCTGGTGCGCACCCAGGACGGCAAGCGCCGCGCCGCGGTGGAGGTGCTGCTGGGCACCGCGACCATCAGCGACATCATCA
AGCGCGGCGACTTCAGTTCGATCAAGGAGATCATGGACAAGTCCCGCGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGCGCGATCAGCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGCACCGGGACAACCCGGCCGCGGCCCAGACCCAGGCCGCCAGCGCCCAGCCGGCAACCCCGGCCGCGCCCG
CGGTGGAAGCCTCCAGCTGGGGCCTGGAACTGGCCCTGGAAGAGATCAAGGCCGAGGACGAGGAAGACAAGAAGGGCGGT
TGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.695

87.75

0.497
  pilU Acinetobacter baylyi ADP1

53.409

88

0.47
  pilU Vibrio cholerae strain A1552

52.125

88.25

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.157

95.25

0.382

Multiple sequence alignment