Detailed information NTG0330130

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EI070_RS01485
• Genome accession: NZ_CP034243
• Coordinates: 312670..313728 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii isolate 09A16CRGN003B
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 307670..318728

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EI070_RS01470 (EI070_01470)	-	308775..309107 (+)	333	WP_000169594.1	hypothetical protein	-
EI070_RS01475 (EI070_01475)	-	309110..309934 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
EI070_RS01480 (EI070_01480)	ponA	309952..312507 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
EI070_RS01485 (EI070_01485)	pilM	312670..313728 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
EI070_RS01490 (EI070_01490)	pilN	313728..314369 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
EI070_RS01495 (EI070_01495)	pilO	314366..315106 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
EI070_RS01500 (EI070_01500)	pilP	315117..315644 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
EI070_RS01505 (EI070_01505)	pilQ	315712..317877 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
EI070_RS01510 (EI070_01510)	aroK	317889..318458 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=330130 EI070_RS01485 WP_002014066.1 312670..313728(+) (pilM) [Acinetobacter baumannii isolate 09A16CRGN003B]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=330130 EI070_RS01485 WP_002014066.1 312670..313728(+) (pilM) [Acinetobacter baumannii isolate 09A16CRGN003B]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423