Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   MCCL_RS06605 Genome accession   NC_011999
Coordinates   1291637..1292608 (-) Length   323 a.a.
NCBI ID   WP_012657092.1    Uniprot ID   B9E6S6
Organism   Macrococcoides caseolyticum JCSC5402     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1286637..1297608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MCCL_RS06575 (MCCL_1181) gcvT 1287334..1288419 (-) 1086 WP_041636415.1 glycine cleavage system aminomethyltransferase GcvT -
  MCCL_RS06580 (MCCL_1182) - 1288557..1289063 (-) 507 WP_012657087.1 shikimate kinase -
  MCCL_RS06585 (MCCL_1183) comGF 1289224..1289676 (-) 453 WP_012657088.1 competence type IV pilus minor pilin ComGF -
  MCCL_RS12080 - 1289621..1289863 (-) 243 WP_133433068.1 hypothetical protein -
  MCCL_RS06590 (MCCL_1184) - 1289850..1290275 (-) 426 WP_012657089.1 type II secretion system protein -
  MCCL_RS06595 (MCCL_1185) comGC 1290268..1290591 (-) 324 WP_041636417.1 competence type IV pilus major pilin ComGC Machinery gene
  MCCL_RS06600 (MCCL_1186) comGB 1290613..1291662 (-) 1050 WP_158298078.1 competence type IV pilus assembly protein ComGB -
  MCCL_RS06605 (MCCL_1187) comGA 1291637..1292608 (-) 972 WP_012657092.1 competence type IV pilus ATPase ComGA Machinery gene
  MCCL_RS06610 - 1292820..1293068 (+) 249 WP_041636023.1 DUF2626 domain-containing protein -
  MCCL_RS06615 (MCCL_1188) - 1293095..1293715 (-) 621 WP_012657093.1 MBL fold metallo-hydrolase -
  MCCL_RS11960 (MCCL_1189) - 1293805..1294044 (+) 240 WP_041636024.1 DUF2759 domain-containing protein -
  MCCL_RS06625 (MCCL_1190) - 1294071..1294382 (-) 312 WP_012657095.1 MTH1187 family thiamine-binding protein -
  MCCL_RS06630 (MCCL_1191) - 1294382..1295350 (-) 969 WP_012657096.1 ROK family glucokinase -
  MCCL_RS06635 - 1295347..1295538 (-) 192 WP_041636025.1 YqgQ family protein -
  MCCL_RS06640 (MCCL_1192) - 1295543..1296991 (-) 1449 WP_012657097.1 rhomboid family protein -
  MCCL_RS06645 (MCCL_1193) - 1296967..1297521 (-) 555 WP_041636026.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 37136.78 Da        Isoelectric Point: 6.0532

>NTDB_id=32884 MCCL_RS06605 WP_012657092.1 1291637..1292608(-) (comGA) [Macrococcoides caseolyticum JCSC5402]
MEKLFNEIIEQAILQSASDIHFIPCDKNVSIKFRVQGDIEEYSDIDDILFKKLLSYIKFTAHLDVSEKNKAQSGIIHFNL
DNLRYNIRASTLPRSLGDEACVLRIIRQSFIDEYQTDDQILFDQMKKSSGIIIISGPTGSGKSTLMYQLVHFAKDTLKRQ
VISIEDPVEQHLDGIIQVNVNEKAEITYQTAIKAILRCDPDIIMLGEIRDSVVAQQVINAGLSGHLVLTTLHANDCIGAL
FRLKEMGINAVDLYQSINLIINQRLIKKRDEKERILAYEFLTKKDIEKYLKNKHINYRTLADILKEMYETNQISQHEFEK
FDL

Nucleotide


Download         Length: 972 bp        

>NTDB_id=32884 MCCL_RS06605 WP_012657092.1 1291637..1292608(-) (comGA) [Macrococcoides caseolyticum JCSC5402]
ATGGAGAAATTATTCAATGAAATAATAGAGCAGGCAATTTTACAAAGTGCATCAGATATACACTTCATTCCTTGTGATAA
AAATGTATCTATTAAATTTAGGGTACAAGGTGATATCGAAGAATATAGTGACATCGACGATATATTATTTAAAAAATTAC
TTTCATATATTAAATTTACAGCACATCTTGATGTATCAGAAAAGAATAAGGCTCAGAGTGGGATAATACATTTTAATCTG
GATAACTTACGATATAATATTCGCGCATCTACTTTACCTCGTTCATTAGGCGATGAAGCATGTGTATTGAGAATCATCAG
ACAAAGTTTTATAGATGAATATCAGACAGATGATCAGATATTGTTCGATCAGATGAAAAAATCAAGCGGTATAATTATTA
TTAGTGGGCCAACTGGAAGTGGTAAGAGTACATTAATGTATCAACTTGTACATTTTGCAAAGGACACATTGAAACGCCAA
GTAATTTCAATAGAAGATCCTGTGGAGCAGCATCTTGACGGTATCATACAAGTTAATGTTAACGAAAAAGCAGAAATAAC
ATATCAAACCGCAATTAAGGCAATCCTTAGATGTGATCCAGATATCATTATGCTAGGTGAAATAAGAGATTCGGTCGTAG
CACAGCAAGTTATCAATGCGGGGCTGAGTGGTCATTTAGTTTTAACGACATTACATGCAAATGATTGTATTGGCGCATTA
TTTCGGTTAAAAGAAATGGGAATTAATGCTGTTGATCTTTATCAAAGTATCAATTTGATAATCAATCAAAGACTGATTAA
AAAAAGAGATGAAAAGGAGCGTATTCTAGCCTATGAATTTCTGACAAAAAAGGATATTGAAAAATATTTAAAGAATAAGC
ACATAAACTATAGAACGTTGGCTGACATACTAAAGGAGATGTATGAAACAAATCAGATTTCACAACATGAATTTGAAAAA
TTTGATCTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9E6S6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

48.923

100

0.492
  comGA Staphylococcus aureus N315

48.923

100

0.492
  comGA Bacillus subtilis subsp. subtilis str. 168

34.77

100

0.375

Multiple sequence alignment