Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   TGR7_RS15155 Genome accession   NC_011901
Coordinates   3164703..3165764 (+) Length   353 a.a.
NCBI ID   WP_012639556.1    Uniprot ID   B8GPU6
Organism   Thioalkalivibrio sulfidiphilus HL-EbGr7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 3166321..3167301 3164703..3165764 flank 557


Gene organization within MGE regions


Location: 3164703..3167301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TGR7_RS15155 (Tgr7_3023) pilM 3164703..3165764 (+) 1062 WP_012639556.1 pilus assembly protein PilM Machinery gene
  TGR7_RS15160 (Tgr7_3024) - 3165764..3166283 (+) 520 Protein_3032 PilN domain-containing protein -
  TGR7_RS15165 (Tgr7_3025) - 3166321..3167301 (+) 981 WP_012637023.1 IS5-like element IS1384 family transposase -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38270.71 Da        Isoelectric Point: 4.5603

>NTDB_id=32755 TGR7_RS15155 WP_012639556.1 3164703..3165764(+) (pilM) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
MIGFSRKKPPLLGIDISSTSVKLVELSQSGGKYRVESYAVEPLPANAVVEKNIQEPEAVGDAIKKAHKRSGSKTRTCAMA
VPSSAVITKVITMPASIKEDEMEGQIQIEADQYIPYALEEVNLDFEVLGPSAKNPETVDVLLSASRSENVELRVSAAELA
GLEPVVMDVEAYAIEHTYPLLLAQMEDVDATGTVAVIDIGATMTSINILSDSKLIYTREQTFGGKQLTEEIMRRYGLSYD
EAGLAKKEGGLPDNYAPEVLEPFKDTMVQQVGRFLQFFYAASQHNHVDQIVLAGGCAAIPGVDELIESRLGTRTLIANPF
GRMSLSTRVNPQRLGNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=32755 TGR7_RS15155 WP_012639556.1 3164703..3165764(+) (pilM) [Thioalkalivibrio sulfidiphilus HL-EbGr7]
GTGATAGGGTTCAGCCGCAAGAAGCCGCCCTTGCTGGGCATCGACATCAGCTCGACCTCGGTCAAGCTGGTGGAACTCAG
CCAGTCCGGCGGCAAATACCGGGTTGAAAGCTACGCCGTTGAGCCGCTGCCGGCCAACGCCGTGGTGGAAAAGAACATCC
AGGAACCCGAGGCCGTGGGCGATGCCATCAAGAAGGCCCACAAGCGTTCCGGGAGCAAGACCCGCACCTGCGCCATGGCC
GTACCCTCCTCCGCCGTGATCACCAAGGTGATCACCATGCCCGCCTCCATCAAGGAGGATGAGATGGAAGGCCAGATCCA
GATCGAGGCGGATCAGTACATCCCCTACGCCCTGGAAGAGGTCAACCTGGATTTCGAGGTGCTGGGCCCCAGTGCCAAGA
ACCCCGAGACCGTGGACGTCCTGCTCTCCGCCTCCCGCAGTGAAAACGTGGAGCTGCGGGTAAGCGCCGCCGAACTGGCC
GGCCTGGAACCCGTGGTCATGGATGTCGAGGCCTATGCCATCGAGCACACCTACCCCCTGCTGCTCGCGCAGATGGAGGA
TGTGGACGCGACGGGCACCGTGGCGGTGATCGACATCGGCGCCACCATGACCAGCATCAACATCCTCAGCGACAGCAAGC
TCATCTACACCCGCGAACAGACCTTCGGTGGCAAGCAGCTCACCGAGGAGATCATGCGCCGCTACGGCCTGTCCTATGAC
GAGGCCGGCCTGGCCAAGAAGGAGGGCGGCCTGCCGGACAACTACGCCCCCGAGGTGCTGGAACCCTTCAAGGACACCAT
GGTGCAGCAGGTGGGTCGTTTCCTGCAGTTCTTCTACGCCGCCAGCCAGCACAACCACGTGGATCAGATCGTACTCGCCG
GAGGCTGCGCCGCGATCCCCGGCGTGGACGAACTGATCGAATCGCGGCTCGGCACCCGCACCCTGATCGCCAACCCCTTC
GGGCGCATGTCCCTGAGTACCCGCGTCAACCCGCAGCGACTCGGCAATGATGCCCCCTCGCTCATGATCGCCTGCGGCCT
GGCCATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8GPU6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

53.39

100

0.535
  comM Acinetobacter nosocomialis M2

53.39

100

0.535
  comM Acinetobacter baylyi ADP1

50

99.15

0.496
  pilM Legionella pneumophila strain ERS1305867

46.398

98.3

0.456

Multiple sequence alignment