Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   EHF40_RS00710 Genome accession   NZ_CP033907
Coordinates   108586..109020 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain Duke-Large     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 103586..114020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHF40_RS00680 (EHF40_00680) - 105255..105620 (+) 366 WP_002986560.1 DUF1033 family protein -
  EHF40_RS00685 (EHF40_00685) comYA 105713..106651 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  EHF40_RS00690 (EHF40_00690) comYB 106587..107621 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EHF40_RS00695 (EHF40_00695) comYC 107623..107949 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EHF40_RS00700 (EHF40_00700) comGD 107924..108352 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EHF40_RS00705 (EHF40_00705) comGE 108309..108593 (+) 285 WP_011528162.1 competence type IV pilus minor pilin ComGE -
  EHF40_RS00710 (EHF40_00710) comYF 108586..109020 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  EHF40_RS00715 (EHF40_00715) comGG 109004..109330 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EHF40_RS00720 (EHF40_00720) comYH 109428..110381 (+) 954 WP_021299429.1 class I SAM-dependent methyltransferase Machinery gene
  EHF40_RS00725 (EHF40_00725) - 110440..111636 (+) 1197 WP_011528164.1 acetate kinase -
  EHF40_RS00730 (EHF40_00730) - 111823..112131 (+) 309 WP_011528165.1 hypothetical protein -
  EHF40_RS00735 (EHF40_00735) proC 112214..112984 (-) 771 WP_002986527.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=326892 EHF40_RS00710 WP_002992738.1 108586..109020(+) (comYF) [Streptococcus pyogenes strain Duke-Large]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=326892 EHF40_RS00710 WP_002992738.1 108586..109020(+) (comYF) [Streptococcus pyogenes strain Duke-Large]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment