Detailed information NTG0326732

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EGM96_RS17305
• Genome accession: NZ_CP033876
• Coordinates: 3578903..3580021 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain WU_164
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3573903..3585021

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGM96_RS17295 (EGM96_17375)	-	3577019..3577711 (-)	693	WP_031959811.1	YggS family pyridoxal phosphate-dependent enzyme	-
EGM96_RS17300 (EGM96_17380)	pilT	3577838..3578875 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EGM96_RS17305 (EGM96_17385)	pilU	3578903..3580021 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EGM96_RS17310 (EGM96_17390)	fur	3580091..3580528 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EGM96_RS17315 (EGM96_17395)	-	3580640..3581038 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EGM96_RS17320 (EGM96_17400)	-	3581093..3581416 (-)	324	WP_000442585.1	RnfH family protein	-
EGM96_RS17325 (EGM96_17405)	-	3581416..3582483 (-)	1068	WP_031959810.1	membrane protein	-
EGM96_RS17330 (EGM96_17415)	-	3582636..3583091 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EGM96_RS17335 (EGM96_17420)	-	3583344..3584192 (-)	849	WP_000336551.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=326732 EGM96_RS17305 WP_000347036.1 3578903..3580021(+) (pilU) [Acinetobacter baumannii strain WU_164]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=326732 EGM96_RS17305 WP_000347036.1 3578903..3580021(+) (pilU) [Acinetobacter baumannii strain WU_164]
ATGGATTTTAATGACCTGCTCAACCTGATGGTTGAAAAGAAATCATCGGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAATGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTACAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCACAGCCAGCTTTATATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392