Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGM96_RS04040 Genome accession   NZ_CP033876
Coordinates   861675..862415 (+) Length   246 a.a.
NCBI ID   WP_000076106.1    Uniprot ID   -
Organism   Acinetobacter baumannii strain WU_164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856675..867415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM96_RS04025 (EGM96_04040) ponA 857262..859817 (-) 2556 WP_031965088.1 penicillin-binding protein PBP1a -
  EGM96_RS04030 (EGM96_04045) pilM 859979..861037 (+) 1059 WP_031961398.1 pilus assembly protein PilM Machinery gene
  EGM96_RS04035 (EGM96_04050) pilN 861037..861678 (+) 642 WP_031961396.1 PilN domain-containing protein Machinery gene
  EGM96_RS04040 (EGM96_04055) pilO 861675..862415 (+) 741 WP_000076106.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGM96_RS04045 (EGM96_04060) pilP 862426..862953 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  EGM96_RS04050 (EGM96_04065) pilQ 863016..865181 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  EGM96_RS04055 (EGM96_04070) aroK 865193..865762 (+) 570 WP_000643712.1 shikimate kinase AroK -
  EGM96_RS04060 (EGM96_04075) aroB 865791..866873 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27805.56 Da        Isoelectric Point: 4.8357

>NTDB_id=326714 EGM96_RS04040 WP_000076106.1 861675..862415(+) (pilO) [Acinetobacter baumannii strain WU_164]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWVFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=326714 EGM96_RS04040 WP_000076106.1 861675..862415(+) (pilO) [Acinetobacter baumannii strain WU_164]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGGTTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTACAGCTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.008

98.374

0.679

Multiple sequence alignment