Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   EGM96_RS04035 Genome accession   NZ_CP033876
Coordinates   861037..861678 (+) Length   213 a.a.
NCBI ID   WP_031961396.1    Uniprot ID   A0A836M414
Organism   Acinetobacter baumannii strain WU_164     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 856037..866678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGM96_RS04015 (EGM96_04030) - 856085..856417 (+) 333 WP_000169602.1 hypothetical protein -
  EGM96_RS04020 (EGM96_04035) - 856420..857244 (+) 825 WP_002019666.1 putative RNA methyltransferase -
  EGM96_RS04025 (EGM96_04040) ponA 857262..859817 (-) 2556 WP_031965088.1 penicillin-binding protein PBP1a -
  EGM96_RS04030 (EGM96_04045) pilM 859979..861037 (+) 1059 WP_031961398.1 pilus assembly protein PilM Machinery gene
  EGM96_RS04035 (EGM96_04050) pilN 861037..861678 (+) 642 WP_031961396.1 PilN domain-containing protein Machinery gene
  EGM96_RS04040 (EGM96_04055) pilO 861675..862415 (+) 741 WP_000076106.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGM96_RS04045 (EGM96_04060) pilP 862426..862953 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  EGM96_RS04050 (EGM96_04065) pilQ 863016..865181 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  EGM96_RS04055 (EGM96_04070) aroK 865193..865762 (+) 570 WP_000643712.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 213 a.a.        Molecular weight: 23931.29 Da        Isoelectric Point: 5.8563

>NTDB_id=326713 EGM96_RS04035 WP_031961396.1 861037..861678(+) (pilN) [Acinetobacter baumannii strain WU_164]
MATINLLPWREELREQRKKQFITLCFGVVVLGITTVFGGWFYFDHKLDDQEQANQLIMSTNQNLDQQLKTLNGLQEQRDA
IIERMKLIQGLQSQRPVVVRLVDELVRVTPPAMYLTKFSRTGDKFTIEGKAESPNTVAELLRNLEASPWYRNAFMNSFLA
NEEKKDKAASSLLPRVEDHYGSFVVTVDLGEMGVATTDDSAKTSTGGSVGAAK

Nucleotide


Download         Length: 642 bp        

>NTDB_id=326713 EGM96_RS04035 WP_031961396.1 861037..861678(+) (pilN) [Acinetobacter baumannii strain WU_164]
ATGGCAACAATTAATTTACTGCCTTGGCGCGAAGAGCTGAGGGAGCAACGAAAAAAACAATTTATAACGTTATGTTTTGG
GGTGGTTGTTTTGGGGATTACAACCGTATTTGGGGGATGGTTTTATTTTGACCATAAATTAGATGATCAAGAGCAAGCGA
ATCAGCTCATTATGAGCACAAATCAGAACCTTGATCAGCAATTAAAAACATTAAACGGCTTACAGGAACAGCGTGATGCC
ATTATTGAACGCATGAAGTTGATCCAAGGTTTGCAGAGTCAACGTCCAGTAGTGGTAAGACTTGTTGATGAGTTAGTTCG
AGTCACACCACCAGCTATGTATTTAACGAAATTTAGCCGAACCGGAGATAAGTTTACGATTGAAGGTAAAGCTGAGAGTC
CAAATACAGTAGCCGAACTACTCCGTAATCTTGAAGCATCTCCTTGGTATCGTAATGCTTTTATGAACTCTTTCTTGGCT
AATGAAGAGAAAAAAGATAAGGCTGCAAGTTCTCTTCTTCCTAGGGTGGAAGACCACTATGGTAGTTTTGTTGTGACTGT
CGATTTGGGGGAAATGGGCGTGGCCACAACGGATGATTCTGCTAAGACATCTACAGGTGGAAGTGTGGGGGCTGCTAAAT
GA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-167)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A836M414

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Acinetobacter baumannii D1279779

98.122

100

0.981
  comN Acinetobacter baylyi ADP1

71.77

98.122

0.704

Multiple sequence alignment