Detailed information NTG0326669

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EGM95_RS16375
• Genome accession: NZ_CP033869
• Coordinates: 3315733..3316851 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347040.1
• Uniprot ID: A0A809JLP7
• Organism: Acinetobacter baumannii strain MRSN15313
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3310733..3321851

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGM95_RS16365 (EGM95_16365)	-	3313849..3314541 (-)	693	WP_004748094.1	YggS family pyridoxal phosphate-dependent enzyme	-
EGM95_RS16370 (EGM95_16370)	pilT	3314668..3315705 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EGM95_RS16375 (EGM95_16375)	pilU	3315733..3316851 (+)	1119	WP_000347040.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EGM95_RS16380 (EGM95_16380)	fur	3316921..3317358 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EGM95_RS16385 (EGM95_16385)	-	3317470..3317868 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EGM95_RS16390 (EGM95_16390)	-	3317923..3318246 (-)	324	WP_000442590.1	RnfH family protein	-
EGM95_RS16395 (EGM95_16395)	-	3318246..3319313 (-)	1068	WP_046693192.1	membrane protein	-
EGM95_RS16405 (EGM95_16405)	-	3319466..3319921 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EGM95_RS16410 (EGM95_16410)	-	3320174..3321022 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41896.10 Da
• Isoelectric Point: 6.6406

>NTDB_id=326669 EGM95_RS16375 WP_000347040.1 3315733..3316851(+) (pilU) [Acinetobacter baumannii strain MRSN15313]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=326669 EGM95_RS16375 WP_000347040.1 3315733..3316851(+) (pilU) [Acinetobacter baumannii strain MRSN15313]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGACAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAGGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACATTACGACAAGCGCCGGATGTCATCTTAATTGGTGAGATTCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGCCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAGGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A809JLP7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392