Detailed information NTG0326641

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EGM95_RS01675
• Genome accession: NZ_CP033869
• Coordinates: 355481..356539 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain MRSN15313
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 350481..361539

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGM95_RS01660 (EGM95_01660)	-	351587..351919 (+)	333	WP_000169601.1	hypothetical protein	-
EGM95_RS01665 (EGM95_01665)	-	351922..352746 (+)	825	WP_086242007.1	putative RNA methyltransferase	-
EGM95_RS01670 (EGM95_01670)	ponA	352764..355319 (-)	2556	WP_000736683.1	penicillin-binding protein PBP1a	-
EGM95_RS01675 (EGM95_01675)	pilM	355481..356539 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
EGM95_RS01680 (EGM95_01680)	pilN	356539..357180 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
EGM95_RS01685 (EGM95_01685)	pilO	357177..357917 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
EGM95_RS01690 (EGM95_01690)	pilP	357928..358455 (+)	528	WP_000695058.1	pilus assembly protein PilP	Machinery gene
EGM95_RS01695 (EGM95_01695)	pilQ	358518..360683 (+)	2166	WP_001017038.1	type IV pilus secretin PilQ family protein	Machinery gene
EGM95_RS01700 (EGM95_01700)	aroK	360695..361264 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=326641 EGM95_RS01675 WP_002014066.1 355481..356539(+) (pilM) [Acinetobacter baumannii strain MRSN15313]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=326641 EGM95_RS01675 WP_002014066.1 355481..356539(+) (pilM) [Acinetobacter baumannii strain MRSN15313]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAATGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCGGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCCTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACTATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCCTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423