Detailed information NTG0326467

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EGY13_RS16040
• Genome accession: NZ_CP033858
• Coordinates: 3205470..3206588 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter sp. FDAARGOS_493
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3200470..3211588

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGY13_RS16030 (EGY13_16040)	-	3203586..3204278 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
EGY13_RS16035 (EGY13_16045)	pilT	3204405..3205442 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EGY13_RS16040 (EGY13_16050)	pilU	3205470..3206588 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EGY13_RS16045 (EGY13_16055)	fur	3206658..3207095 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EGY13_RS16050 (EGY13_16060)	-	3207207..3207605 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EGY13_RS16055 (EGY13_16065)	-	3207660..3207983 (-)	324	WP_000442588.1	RnfH family protein	-
EGY13_RS16060 (EGY13_16070)	-	3207983..3209050 (-)	1068	WP_002019572.1	hypothetical protein	-
EGY13_RS16070 (EGY13_16080)	-	3209203..3209658 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EGY13_RS16075 (EGY13_16085)	-	3209911..3210759 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=326467 EGY13_RS16040 WP_000347034.1 3205470..3206588(+) (pilU) [Acinetobacter sp. FDAARGOS_493]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=326467 EGY13_RS16040 WP_000347034.1 3205470..3206588(+) (pilU) [Acinetobacter sp. FDAARGOS_493]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACTAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39