Detailed information NTG0326043

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: EGX80_RS05650
• Genome accession: NZ_CP033815
• Coordinates: 1044140..1044574 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain FDAARGOS_514
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1039140..1049574

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGX80_RS05620 (EGX80_05630)	-	1040809..1041174 (+)	366	WP_002986560.1	DUF1033 family protein	-
EGX80_RS05625 (EGX80_05635)	comYA	1041267..1042205 (+)	939	WP_011054114.1	competence type IV pilus ATPase ComGA	Machinery gene
EGX80_RS05630 (EGX80_05640)	comYB	1042141..1043175 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
EGX80_RS05635 (EGX80_05645)	comYC	1043177..1043503 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
EGX80_RS05640 (EGX80_05650)	comGD	1043478..1043906 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
EGX80_RS05645 (EGX80_05655)	comGE	1043863..1044147 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
EGX80_RS05650 (EGX80_05660)	comYF	1044140..1044574 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
EGX80_RS05655 (EGX80_05665)	comGG	1044558..1044884 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
EGX80_RS05660 (EGX80_05670)	comYH	1044982..1045935 (+)	954	WP_011054117.1	class I SAM-dependent methyltransferase	Machinery gene
EGX80_RS05665 (EGX80_05675)	-	1045994..1047190 (+)	1197	WP_010921803.1	acetate kinase	-
EGX80_RS05670 (EGX80_05680)	-	1047376..1047684 (+)	309	WP_011054118.1	hypothetical protein	-
EGX80_RS05675 (EGX80_05685)	proC	1047767..1048537 (-)	771	WP_002992745.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=326043 EGX80_RS05650 WP_002992738.1 1044140..1044574(+) (comYF) [Streptococcus pyogenes strain FDAARGOS_514]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=326043 EGX80_RS05650 WP_002992738.1 1044140..1044574(+) (comYF) [Streptococcus pyogenes strain FDAARGOS_514]
TTGAGTAAACAATTAAGTAATATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGCTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396