Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HAPS_RS09770 Genome accession   NC_011852
Coordinates   1974964..1975416 (-) Length   150 a.a.
NCBI ID   WP_010787008.1    Uniprot ID   B8F829
Organism   Glaesserella parasuis SH0165     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1969964..1980416
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HAPS_RS09750 (HAPS_2009) - 1970734..1971402 (-) 669 WP_015940025.1 prepilin peptidase -
  HAPS_RS09755 (HAPS_2010) pilC 1971399..1972595 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  HAPS_RS09760 (HAPS_2011) pilB 1972588..1973973 (-) 1386 WP_015940026.1 GspE/PulE family protein Machinery gene
  HAPS_RS09765 (HAPS_2012) - 1974326..1974691 (-) 366 WP_015940027.1 endonuclease domain-containing protein -
  HAPS_RS09770 (HAPS_2013) pilA 1974964..1975416 (-) 453 WP_010787008.1 pilin Machinery gene
  HAPS_RS09775 (HAPS_2014) radA 1975604..1976980 (+) 1377 WP_010787009.1 DNA repair protein RadA -
  HAPS_RS09780 (HAPS_2015) gmk 1977033..1977659 (-) 627 WP_010787010.1 guanylate kinase -
  HAPS_RS09785 (HAPS_2016) - 1977728..1978699 (-) 972 WP_012621529.1 IS110 family transposase -

Sequence

Protein


Download         Length: 150 a.a.        Molecular weight: 16138.74 Da        Isoelectric Point: 9.0811

>NTDB_id=32584 HAPS_RS09770 WP_010787008.1 1974964..1975416(-) (pilA) [Glaesserella parasuis SH0165]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVRADKSDVPDAKYLKSISVASGVITVAGKGNIDGYGYTMTPKFANNNITWATSCQGADISLFPENFCRQ

Nucleotide


Download         Length: 453 bp        

>NTDB_id=32584 HAPS_RS09770 WP_010787008.1 1974964..1975416(-) (pilA) [Glaesserella parasuis SH0165]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTAATGATCGTGATCGCCATCATTGC
CATTTTAGCAACAATAGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCAGCTCTCTCTGAGTTATTAAGTGCTTCCG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCGAGCAGATAAATCCGATGTCCCTGACGCGAAATACCTTAAATCTATTTCCGTCGCTTCGGGGGTAATCACCGT
GGCAGGTAAAGGCAATATTGATGGCTACGGCTACACAATGACTCCGAAATTCGCTAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGAGAATTTCTGTAGGCAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B8F829

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

98.667

100

0.987
  pilA Haemophilus influenzae Rd KW20

54.054

98.667

0.533
  pilA Haemophilus influenzae 86-028NP

53.378

98.667

0.527

Multiple sequence alignment