Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FLK62_RS00895 Genome accession   NZ_CP041334
Coordinates   185396..185860 (+) Length   154 a.a.
NCBI ID   WP_176443367.1    Uniprot ID   -
Organism   Glaesserella parasuis strain HPS412     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 180396..190860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FLK62_RS00875 (FLK62_00870) gpt 181233..181712 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  FLK62_RS00880 (FLK62_00875) - 182113..183084 (+) 972 WP_176443366.1 transposase -
  FLK62_RS00885 (FLK62_00880) gmk 183153..183779 (+) 627 WP_021113158.1 guanylate kinase -
  FLK62_RS00890 (FLK62_00885) radA/sms 183832..185208 (-) 1377 WP_005711784.1 DNA repair protein RadA Machinery gene
  FLK62_RS00895 (FLK62_00890) pilA 185396..185860 (+) 465 WP_176443367.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  FLK62_RS00900 (FLK62_00895) pilB 185908..187293 (+) 1386 WP_176443368.1 GspE/PulE family protein Machinery gene
  FLK62_RS00905 (FLK62_00900) pilC 187286..188482 (+) 1197 WP_149350581.1 type II secretion system F family protein Machinery gene
  FLK62_RS00910 (FLK62_00905) - 188479..189147 (+) 669 WP_035491631.1 prepilin peptidase -
  FLK62_RS00915 (FLK62_00910) dapE 189231..190364 (+) 1134 WP_176443369.1 succinyl-diaminopimelate desuccinylase -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16375.95 Da        Isoelectric Point: 8.7719

>NTDB_id=325425 FLK62_RS00895 WP_176443367.1 185396..185860(+) (pilA) [Glaesserella parasuis strain HPS412]
MKKIRLIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLSASASYKSDVEICIYNVGDKKISECSSGKN
GVQADKTDVADAKYLKSISVASGTITVEGKGSIDGYGYTMTPKFANNNITWATSCQGADISLFPANFCGTKATQ

Nucleotide


Download         Length: 465 bp        

>NTDB_id=325425 FLK62_RS00895 WP_176443367.1 185396..185860(+) (pilA) [Glaesserella parasuis strain HPS412]
ATGAAAAAAATCAGGTTAATTCGACCGCTTGCAAAAGCCTTTACCTTAATTGAGTTGATGATCGTGATCGCCATCATTGC
TATTTTAGCCACGATTGCAATCCCGTCTTACACCAGTTACACCCAAAAGGCTGCACTCTCTGAGTTATTAAGTGCTTCTG
CATCTTATAAATCCGATGTGGAAATTTGTATTTATAACGTAGGGGATAAAAAAATCTCGGAATGTAGTTCAGGTAAAAAT
GGCGTTCAAGCGGATAAAACCGATGTTGCCGATGCGAAATATCTTAAATCTATTTCCGTCGCTTCGGGGACAATCACCGT
AGAAGGCAAAGGCAGCATTGATGGCTACGGCTACACAATGACTCCGAAATTCGCTAATAACAACATCACTTGGGCAACAA
GCTGTCAAGGGGCTGACATTAGTTTGTTCCCTGCGAATTTTTGTGGGACAAAAGCCACACAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

95.425

99.351

0.948
  pilA Haemophilus influenzae Rd KW20

54.667

97.403

0.532
  pilA Haemophilus influenzae 86-028NP

53.691

96.753

0.519

Multiple sequence alignment