Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FKM99_RS23015 Genome accession   NZ_CP041202
Coordinates   2842973..2843407 (+) Length   144 a.a.
NCBI ID   WP_025501252.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain Vb0624     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2837973..2848407
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FKM99_RS23000 (FKM99_23000) pdhR 2840006..2840773 (-) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  FKM99_RS23005 (FKM99_23005) ampD 2841179..2841730 (-) 552 WP_005462574.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FKM99_RS23010 (FKM99_23010) nadC 2841823..2842710 (+) 888 WP_141180406.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FKM99_RS23015 (FKM99_23015) pilA 2842973..2843407 (+) 435 WP_025501252.1 type IV pilin protein Machinery gene
  FKM99_RS23020 (FKM99_23020) pilB 2843407..2845092 (+) 1686 WP_141180407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FKM99_RS23025 (FKM99_23025) pilC 2845116..2846339 (+) 1224 WP_005479682.1 type II secretion system F family protein Machinery gene
  FKM99_RS23030 (FKM99_23030) pilD 2846404..2847273 (+) 870 WP_141180408.1 A24 family peptidase Machinery gene
  FKM99_RS23035 (FKM99_23035) coaE 2847274..2847888 (+) 615 WP_141180409.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 14846.81 Da        Isoelectric Point: 8.4659

>NTDB_id=325072 FKM99_RS23015 WP_025501252.1 2842973..2843407(+) (pilA) [Vibrio parahaemolyticus strain Vb0624]
MKNSKQKKQQGFTLIELMIVVAIIGVLSAVAIPAYKDYVAKSAVTSAVGTLKSLLTNADLYTQNNTTATSDLGDIGGNAT
MNALGNITASIEGAGAASNAKSTLKFVFNTGNAAAGDIEYARTPNTPWGCTNNTNPKVSIDSCP

Nucleotide


Download         Length: 435 bp        

>NTDB_id=325072 FKM99_RS23015 WP_025501252.1 2842973..2843407(+) (pilA) [Vibrio parahaemolyticus strain Vb0624]
ATGAAAAACAGTAAACAGAAAAAACAGCAAGGTTTTACGTTAATTGAATTGATGATTGTGGTAGCAATTATTGGGGTGTT
ATCAGCAGTCGCAATCCCTGCATATAAAGACTATGTCGCAAAGTCAGCTGTAACCTCTGCTGTGGGTACTTTAAAGTCAT
TACTTACTAATGCTGATTTATATACTCAGAACAACACCACAGCTACTTCCGACTTAGGAGACATCGGTGGGAACGCTACT
ATGAACGCCCTTGGCAATATTACCGCATCAATTGAAGGTGCTGGAGCAGCCTCAAACGCAAAATCTACACTCAAATTTGT
ATTTAATACTGGCAATGCTGCAGCAGGTGACATCGAATATGCTAGGACACCAAACACACCCTGGGGCTGTACAAATAATA
CTAACCCAAAAGTATCTATTGATAGCTGCCCATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio parahaemolyticus RIMD 2210633

46.897

100

0.472
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.667

100

0.403
  pilA Vibrio cholerae strain A1552

38.667

100

0.403
  pilA Vibrio cholerae C6706

38.667

100

0.403