Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   EFV54_RS05840 Genome accession   NZ_CP033607
Coordinates   1096988..1098310 (-) Length   440 a.a.
NCBI ID   WP_010905751.1    Uniprot ID   Q9CGK6
Organism   Lactococcus lactis strain IL1403     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1091988..1103310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EFV54_RS05810 (EFV54_05810) - 1093131..1094099 (+) 969 WP_003130649.1 PhoH family protein -
  EFV54_RS05815 (EFV54_05815) - 1094157..1094636 (+) 480 WP_003130650.1 NUDIX hydrolase -
  EFV54_RS05820 (EFV54_05820) ybeY 1094788..1095276 (+) 489 WP_003130652.1 rRNA maturation RNase YbeY -
  EFV54_RS05825 (EFV54_05825) - 1095260..1095715 (+) 456 WP_003130653.1 diacylglycerol kinase -
  EFV54_RS05830 (EFV54_05830) - 1095729..1096307 (+) 579 WP_003130655.1 nucleotidyltransferase family protein -
  EFV54_RS12400 comFC 1096341..1096745 (-) 405 WP_003130657.1 ComF family protein Machinery gene
  EFV54_RS05840 (EFV54_05840) comFA 1096988..1098310 (-) 1323 WP_010905751.1 DEAD/DEAH box helicase Machinery gene
  EFV54_RS05845 (EFV54_05845) - 1098367..1098996 (+) 630 WP_021214545.1 YigZ family protein -
  EFV54_RS05850 (EFV54_05850) - 1099130..1099627 (+) 498 WP_003130662.1 VanZ family protein -
  EFV54_RS05855 (EFV54_05855) - 1099675..1100694 (+) 1020 WP_003130663.1 DUF475 domain-containing protein -
  EFV54_RS05860 (EFV54_05860) - 1100804..1101556 (+) 753 WP_010905753.1 bifunctional metallophosphatase/5'-nucleotidase -
  EFV54_RS05865 (EFV54_05865) glyQ 1101841..1102794 (+) 954 WP_003130667.1 glycine--tRNA ligase subunit alpha -

Sequence

Protein


Download         Length: 440 a.a.        Molecular weight: 50233.77 Da        Isoelectric Point: 10.0565

>NTDB_id=324366 EFV54_RS05840 WP_010905751.1 1096988..1098310(-) (comFA) [Lactococcus lactis strain IL1403]
MSTNQEKLFGRLLLKNDILQLIKNTDKISVSKIFSNFLLETKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDDFLYFLPQKNFPKKSYINWSGKLTENQKSISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSRDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYV
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPRRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGCFS

Nucleotide


Download         Length: 1323 bp        

>NTDB_id=324366 EFV54_RS05840 WP_010905751.1 1096988..1098310(-) (comFA) [Lactococcus lactis strain IL1403]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTGCAACTTATAAAAAATACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAACGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGATTTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCTGGAAAACTGACAGAGAATCAAAAATCAATTTCAAATGCCCTTTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAGCAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACGTGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATGTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGCGTCGTTTTCACAATTTCCCTTTAGTGGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACATCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGATGTTTTTCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(114-233)

Helicase conserved C-terminal domain (Helicase_C)

(300-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CGK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Lactococcus lactis subsp. cremoris KW2

74

90.909

0.673
  comFA/cflA Streptococcus mitis SK321

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae Rx1

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae D39

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae R6

52.284

89.545

0.468
  comFA/cflA Streptococcus pneumoniae TIGR4

52.284

89.545

0.468
  comFA/cflA Streptococcus mitis NCTC 12261

51.523

89.545

0.461
  comFA Bacillus subtilis subsp. subtilis str. 168

38.106

98.409

0.375

Multiple sequence alignment