Detailed information NTG0324032

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EEB07_RS02260
• Genome accession: NZ_CP033576
• Coordinates: 438636..439250 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain NY12-2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433636..444250

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EEB07_RS02230 (EEB07_02230)	rpsT	433939..434205 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
EEB07_RS02235 (EEB07_02235)	holA	434168..435263 (-)	1096	Protein_440	DNA polymerase III subunit delta	-
EEB07_RS02240 (EEB07_02240)	-	435303..435454 (-)	152	Protein_441	hypothetical protein	-
EEB07_RS02245 (EEB07_02245)	-	435495..435629 (+)	135	WP_003152870.1	YqzM family protein	-
EEB07_RS02250 (EEB07_02250)	comEC	435648..437999 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EEB07_RS02255 (EEB07_02255)	-	438000..438569 (-)	570	WP_003152868.1	ComE operon protein 2	-
EEB07_RS02260 (EEB07_02260)	comEA	438636..439250 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
EEB07_RS02265 (EEB07_02265)	comER	439309..440130 (+)	822	WP_014305449.1	late competence protein ComER	-
EEB07_RS02270 (EEB07_02270)	-	440199..440936 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
EEB07_RS02275 (EEB07_02275)	rsfS	440933..441289 (-)	357	WP_003152864.1	ribosome silencing factor	-
EEB07_RS02280 (EEB07_02280)	yqeK	441286..441867 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EEB07_RS02285 (EEB07_02285)	-	441857..442426 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
EEB07_RS02290 (EEB07_02290)	yhbY	442437..442727 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
EEB07_RS02295 (EEB07_02295)	aroE	442721..443563 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=324032 EEB07_RS02260 WP_013352942.1 438636..439250(-) (comEA) [Bacillus velezensis strain NY12-2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=324032 EEB07_RS02260 WP_013352942.1 438636..439250(-) (comEA) [Bacillus velezensis strain NY12-2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363