Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   EEB07_RS02250 Genome accession   NZ_CP033576
Coordinates   435648..437999 (-) Length   783 a.a.
NCBI ID   WP_025649830.1    Uniprot ID   A0A411A869
Organism   Bacillus velezensis strain NY12-2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 430648..442999
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEB07_RS02215 (EEB07_02215) - 431014..431352 (-) 339 WP_017418178.1 YqxA family protein -
  EEB07_RS02220 (EEB07_02220) - 431369..432562 (-) 1194 WP_007612673.1 stage II sporulation protein P -
  EEB07_RS02225 (EEB07_02225) gpr 432630..433736 (-) 1107 WP_003152878.1 GPR endopeptidase -
  EEB07_RS02230 (EEB07_02230) rpsT 433939..434205 (+) 267 WP_003152876.1 30S ribosomal protein S20 -
  EEB07_RS02235 (EEB07_02235) holA 434168..435263 (-) 1096 Protein_440 DNA polymerase III subunit delta -
  EEB07_RS02240 (EEB07_02240) - 435303..435454 (-) 152 Protein_441 hypothetical protein -
  EEB07_RS02245 (EEB07_02245) - 435495..435629 (+) 135 WP_003152870.1 YqzM family protein -
  EEB07_RS02250 (EEB07_02250) comEC 435648..437999 (-) 2352 WP_025649830.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EEB07_RS02255 (EEB07_02255) - 438000..438569 (-) 570 WP_003152868.1 ComE operon protein 2 -
  EEB07_RS02260 (EEB07_02260) comEA 438636..439250 (-) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  EEB07_RS02265 (EEB07_02265) comER 439309..440130 (+) 822 WP_014305449.1 late competence protein ComER -
  EEB07_RS02270 (EEB07_02270) - 440199..440936 (-) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  EEB07_RS02275 (EEB07_02275) rsfS 440933..441289 (-) 357 WP_003152864.1 ribosome silencing factor -
  EEB07_RS02280 (EEB07_02280) yqeK 441286..441867 (-) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EEB07_RS02285 (EEB07_02285) - 441857..442426 (-) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  EEB07_RS02290 (EEB07_02290) yhbY 442437..442727 (-) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 783 a.a.        Molecular weight: 86725.67 Da        Isoelectric Point: 8.9055

>NTDB_id=324030 EEB07_RS02250 WP_025649830.1 435648..437999(-) (comEC) [Bacillus velezensis strain NY12-2]
MKYKYLLLPLAAVSATAGIAAAHVFWVLLLFLLYLLFIMIKTKQHAPVIVCLVSFCLYFFLYTVCDAANVTRYQAGSYTE
QVVITNIPKVDGAKMSAVIRTHDKEKWAASYKIRSLEEKRLIEQLEPGMRCTFTGSLEQPAHATVPGGFDYKEYLYSQQI
HWLFSVTSIQQCEKSKQPLFKLLNIRKNLISIIRNHVPESSAGIVEALTLGERFSIEDDILSAYQNLGVVHLMAISGMHV
GLITAGLFYALIRIGLTREKAGILLLLFLPVYTLLSGAAPSVLRASLMLGFYIAGTLVKRGIHSSAALSLSYLLLLLFNP
YFLWQAGFQLSFAVSASLILSSSILKKAGKSRLAGLAMASFIAELSSLPFLLYHFQQISLVSFPMNMVMVPFYTLFVIPV
SVIGFLLLLLSGQMGECLFDMFDLVMKPVHDFITYAASVDLFTMIVSKPDFLSLLLLAVSVFTLFAALEKGGFLKLRKSV
LFFCAVLAYLICRPYFSPWGEADMLDIGQGDSLFISAPHRKGTVMVDTGGVIAYPGESWKEKRHPYSIGEKVLIPFLNGK
GVKKLDALILTHADQDHIGEAGVLIKNHRVKRLIVPVGFVKEPKDQNILNMAKENNIPVAEAKRGDTITAGDLQFQVLSP
ESSDGRSKNDSSLVLWTVLSGVSWLLTGDLESDGETEVLKTYPNLKADILKAGHHGSKSSTSEAFLKQLQPEAALISAGK
ENRYHHPHEEVLDRLKAYSVNVLRTDISGTIQYRFKKGAGTFSVFPPYDIEEIRAQEVKKTAD

Nucleotide


Download         Length: 2352 bp        

>NTDB_id=324030 EEB07_RS02250 WP_025649830.1 435648..437999(-) (comEC) [Bacillus velezensis strain NY12-2]
ATGAAATATAAATACCTTCTTCTGCCTCTGGCGGCGGTTTCTGCAACTGCGGGAATTGCCGCCGCTCATGTCTTCTGGGT
TCTGCTCCTTTTTCTTCTGTATCTTCTCTTTATTATGATAAAAACAAAGCAGCATGCTCCGGTTATTGTCTGCCTCGTTT
CTTTTTGTCTTTATTTCTTTCTTTATACGGTTTGTGACGCTGCGAATGTAACGCGGTATCAGGCCGGCAGTTATACTGAA
CAGGTCGTCATCACTAACATTCCGAAGGTTGACGGAGCGAAAATGTCAGCCGTTATCCGTACACATGACAAGGAAAAATG
GGCGGCTTCGTACAAAATCCGGTCTCTTGAGGAAAAGAGACTCATTGAACAGCTTGAACCGGGGATGCGCTGCACGTTTA
CAGGCTCTCTGGAGCAGCCTGCACATGCGACGGTTCCCGGAGGTTTTGATTATAAGGAATATCTTTACTCTCAGCAGATT
CACTGGTTATTTTCCGTGACTTCCATTCAGCAGTGTGAAAAATCCAAACAGCCGCTGTTTAAACTGCTGAACATCAGAAA
AAATTTGATTTCGATCATTCGGAATCACGTGCCTGAATCTTCCGCCGGAATTGTTGAAGCGCTGACCTTAGGTGAAAGAT
TTTCTATAGAGGACGATATACTGAGTGCATATCAAAATTTGGGAGTCGTTCATTTAATGGCGATTTCCGGAATGCATGTC
GGTCTTATTACGGCGGGATTATTTTATGCTCTGATCAGAATCGGGCTGACAAGAGAAAAAGCGGGAATTTTGCTGCTGTT
GTTTTTGCCGGTGTATACGCTGCTGAGCGGTGCCGCCCCATCCGTATTGCGCGCATCCCTCATGCTGGGATTTTATATCG
CCGGAACTCTTGTTAAACGCGGCATTCATTCCTCCGCTGCATTGTCCCTGTCTTATCTGCTGCTCCTGCTGTTTAATCCT
TACTTCCTTTGGCAGGCGGGCTTCCAGCTTTCCTTTGCGGTAAGCGCCTCTTTAATTCTGTCATCCTCCATTTTAAAGAA
AGCAGGGAAAAGCAGACTTGCCGGGCTTGCGATGGCTTCATTCATTGCGGAGCTCAGCTCACTTCCGTTTCTTCTCTATC
ATTTTCAGCAAATTTCACTTGTCAGTTTTCCGATGAATATGGTGATGGTGCCATTTTATACGTTATTTGTCATTCCGGTT
TCTGTCATCGGTTTCCTTCTTCTTTTACTTTCAGGGCAGATGGGAGAATGTTTGTTTGATATGTTCGACCTTGTGATGAA
GCCTGTACATGATTTCATTACATATGCGGCATCCGTTGATTTATTTACTATGATTGTGTCAAAGCCTGACTTTCTTTCCC
TTCTTCTGCTTGCGGTTTCCGTTTTTACTCTTTTTGCGGCTTTGGAAAAGGGAGGTTTTTTAAAACTCAGGAAATCGGTT
CTTTTTTTCTGCGCGGTTTTGGCTTATTTAATATGCCGTCCGTATTTCAGTCCATGGGGAGAAGCGGATATGCTTGATAT
CGGGCAGGGAGACTCACTGTTTATAAGCGCGCCGCACCGCAAAGGGACCGTAATGGTTGATACAGGGGGAGTGATTGCTT
ATCCCGGAGAATCATGGAAAGAAAAACGCCACCCGTATTCTATCGGCGAGAAGGTTTTGATTCCGTTTTTAAACGGAAAA
GGGGTGAAAAAGCTGGATGCGCTGATTTTAACCCATGCGGATCAAGATCACATCGGAGAAGCCGGAGTGTTAATCAAAAA
TCATAGAGTCAAACGGTTAATTGTCCCCGTGGGATTCGTAAAAGAACCGAAGGATCAGAACATATTAAATATGGCGAAAG
AAAACAACATTCCCGTTGCCGAAGCAAAGCGGGGCGACACCATTACAGCCGGTGATCTTCAGTTTCAGGTGCTGTCTCCG
GAGTCGTCTGACGGAAGGAGTAAAAATGATTCGTCACTGGTGCTTTGGACGGTTTTAAGCGGAGTGAGCTGGCTTTTGAC
GGGAGATTTAGAATCGGACGGCGAAACGGAAGTGCTGAAAACGTATCCGAATCTGAAGGCTGATATATTGAAGGCGGGTC
ATCACGGCAGCAAAAGCTCAACGAGTGAAGCCTTTTTGAAACAGCTTCAGCCGGAAGCGGCGCTGATTTCAGCAGGAAAA
GAGAATCGATACCATCATCCGCATGAAGAAGTGCTGGATCGTTTGAAGGCGTACTCTGTCAATGTGCTTCGCACCGATAT
CAGCGGAACGATTCAATACAGATTTAAAAAAGGCGCCGGAACGTTTTCCGTCTTCCCTCCATATGATATAGAAGAAATCA
GGGCGCAAGAAGTAAAAAAGACTGCCGATTGA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(25-171)

Competence protein (Competence)

(208-469)

Metallo-beta-lactamase superfamily (Lactamase_B)

(508-719)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A869

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

57.05

98.723

0.563

Multiple sequence alignment