Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D8668_RS02145 Genome accession   NZ_CP033439
Coordinates   470628..471692 (+) Length   354 a.a.
NCBI ID   WP_108720490.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain SP4528     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 465628..476692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D8668_RS02135 (D8668_02135) - 466608..467876 (+) 1269 WP_003095842.1 malic enzyme -
  D8668_RS02140 (D8668_02140) - 467972..470443 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  D8668_RS02145 (D8668_02145) pilM 470628..471692 (+) 1065 WP_108720490.1 type IV pilus assembly protein PilM Machinery gene
  D8668_RS02150 (D8668_02150) pilN 471692..472288 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  D8668_RS02155 (D8668_02155) pilO 472285..472908 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO -
  D8668_RS02160 (D8668_02160) pilP 472905..473429 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP -
  D8668_RS02165 (D8668_02165) pilQ 473483..475615 (+) 2133 WP_033989912.1 type 4a pilus secretin PilQ Machinery gene
  D8668_RS02170 (D8668_02170) aroK 475627..476145 (+) 519 WP_003095833.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37968.51 Da        Isoelectric Point: 4.5405

>NTDB_id=323750 D8668_RS02145 WP_108720490.1 470628..471692(+) (pilM) [Pseudomonas aeruginosa strain SP4528]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGLFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=323750 D8668_RS02145 WP_108720490.1 470628..471692(+) (pilM) [Pseudomonas aeruginosa strain SP4528]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCGCGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGGCTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAATTCTTCTTCGCCGCCGGATTGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463

Multiple sequence alignment