Detailed information NTG0323472

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JL719_RS22415
• Genome accession: NZ_CP033381
• Coordinates: 4919201..4920262 (-)
• Length: 353 a.a.
• NCBI ID: WP_020483743.1
• Uniprot ID: A0A2N0V797
• Organism: Methylomonas sp. LW13
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4914201..4925262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JL719_RS22390 (U737_22590)	lptM	4914939..4915064 (-)	126	WP_084153286.1	LPS translocon maturation chaperone LptM	-
JL719_RS22395 (U737_22595)	pilQ	4915206..4917449 (-)	2244	WP_033156770.1	type IV pilus secretin PilQ	-
JL719_RS22400 (U737_22600)	-	4917452..4918009 (-)	558	WP_084153287.1	pilus assembly protein PilP	-
JL719_RS22405 (U737_22605)	-	4918006..4918632 (-)	627	WP_033156771.1	type 4a pilus biogenesis protein PilO	-
JL719_RS22410 (U737_22610)	-	4918629..4919201 (-)	573	WP_033156772.1	PilN domain-containing protein	-
JL719_RS22415 (U737_22615)	pilM	4919201..4920262 (-)	1062	WP_020483743.1	pilus assembly protein PilM	Machinery gene
JL719_RS22420 (U737_22620)	-	4920473..4922836 (+)	2364	WP_033156773.1	penicillin-binding protein 1A	-
JL719_RS22425 (U737_22625)	rpmE	4922908..4923108 (-)	201	WP_081607938.1	50S ribosomal protein L31	-
JL719_RS22430 (U737_22630)	-	4923404..4924783 (-)	1380	WP_084153288.1	AmpG family muropeptide MFS transporter	-

Sequence

Protein

• Length: 353 a.a.
• Molecular weight: 37937.42 Da
• Isoelectric Point: 4.7094

>NTDB_id=323472 JL719_RS22415 WP_020483743.1 4919201..4920262(-) (pilM) [Methylomonas sp. LW13]
MSWLSRNPSAVLGVDISTAAVKLLELSRVGARYRVESYSVAPLPQDAIVDKNITNIEQIGAVIKAAVKQSGTRAKQASVA
VAGSSVMTKIISMPASLSDRDMEEQIMVEADQYIPYSLDEVNLDFEVQGITKNNPDMVDVLLAASRRENIEDRVAALAYA
GLKAVVVDVEAFAMENAFSLLADQLPEGAGNMTVAIADIGATMTSLNILHEGKTVYTREQGFGGKQLTEEIQRRYGLSYE
EAGLAKKHGGLPDNYVTDVLEPFKKAMLQQIARSLQFFVSSSANRGVDALVLAGGCASIAGLDKLVERDLGIPSYIANPF
INMALSNRVKPQSLSNDTPAMMIACGLALRSFD

Nucleotide

• Length: 1062 bp

>NTDB_id=323472 JL719_RS22415 WP_020483743.1 4919201..4920262(-) (pilM) [Methylomonas sp. LW13]
ATGAGCTGGTTGAGCAGGAATCCGTCGGCTGTGCTGGGTGTGGATATTAGTACAGCTGCGGTTAAATTGTTGGAATTGAG
CCGGGTCGGTGCTCGTTACCGGGTAGAAAGTTACTCGGTGGCGCCCTTACCTCAAGATGCGATAGTCGATAAGAACATCA
CCAATATAGAGCAAATCGGCGCGGTAATAAAAGCGGCGGTCAAGCAATCCGGTACTCGCGCAAAGCAGGCGAGCGTTGCG
GTTGCGGGTTCGTCGGTGATGACCAAAATCATTTCGATGCCCGCCTCCTTGTCTGATAGGGACATGGAGGAGCAGATCAT
GGTAGAAGCGGATCAGTACATTCCTTATTCGCTGGACGAAGTCAATCTCGATTTCGAAGTCCAGGGCATCACCAAAAATA
ACCCGGATATGGTTGATGTGTTGCTGGCGGCTTCTCGACGGGAAAACATTGAAGACAGGGTGGCTGCGCTGGCCTATGCC
GGCCTGAAGGCTGTCGTCGTCGATGTGGAAGCTTTCGCGATGGAAAATGCGTTTTCCTTGTTGGCGGATCAGTTACCGGA
AGGTGCCGGCAATATGACTGTTGCGATTGCCGACATTGGCGCAACCATGACCTCTCTGAACATTCTGCATGAAGGTAAAA
CTGTTTATACCCGGGAGCAGGGCTTTGGCGGAAAACAGCTAACTGAAGAGATTCAGCGCCGCTATGGCCTGTCGTATGAA
GAAGCCGGTTTGGCCAAGAAGCACGGCGGCTTGCCGGATAATTACGTCACGGATGTGCTGGAGCCCTTCAAAAAAGCCAT
GTTGCAGCAAATCGCCAGATCGTTGCAATTCTTTGTTTCCTCTAGCGCCAATCGCGGCGTCGATGCCTTGGTACTTGCCG
GTGGTTGCGCGTCAATCGCGGGTTTGGACAAATTGGTGGAACGCGACCTCGGCATCCCGTCTTACATTGCCAATCCATTT
ATCAATATGGCGCTTTCTAATCGGGTCAAACCGCAAAGTCTGAGCAACGATACGCCTGCGATGATGATAGCGTGCGGTTT
AGCGCTAAGGAGTTTCGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (13-352)

3D structure

• AlphaFold DB: A0A2N0V797

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	51.695	100	0.518
comM	Acinetobacter nosocomialis M2	51.695	100	0.518
comM	Acinetobacter baylyi ADP1	50	100	0.501
pilM	Legionella pneumophila strain ERS1305867	40.793	100	0.408