Detailed information NTG0032288

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: VS_RS12020
• Genome accession: NC_011753
• Coordinates: 2742960..2743829 (+)
• Length: 289 a.a.
• NCBI ID: WP_012604745.1
• Uniprot ID: -
• Organism: Vibrio atlanticus
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2737960..2748829

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VS_RS12000 (VS_2546)	nadC	2738409..2739299 (+)	891	WP_012604742.1	carboxylating nicotinate-nucleotide diphosphorylase	-
VS_RS12005 (VS_2547)	pilA	2739554..2739964 (+)	411	WP_009847601.1	pilin	Machinery gene
VS_RS12010 (VS_2548)	pilB	2739964..2741649 (+)	1686	WP_041472903.1	type IV-A pilus assembly ATPase PilB	Machinery gene
VS_RS12015 (VS_2549)	pilC	2741665..2742891 (+)	1227	WP_012604744.1	type II secretion system F family protein	Machinery gene
VS_RS12020 (VS_2550)	pilD	2742960..2743829 (+)	870	WP_012604745.1	prepilin peptidase	Machinery gene
VS_RS12025	coaE	2743832..2744453 (+)	622	Protein_2349	dephospho-CoA kinase	-
VS_RS12030 (VS_2553)	zapD	2744480..2745220 (+)	741	WP_012604748.1	cell division protein ZapD	-
VS_RS12035 (VS_2554)	yacG	2745301..2745498 (+)	198	WP_012604749.1	DNA gyrase inhibitor YacG	-
VS_RS12040 (VS_2555)	rplS	2745814..2746167 (-)	354	WP_009847607.1	50S ribosomal protein L19	-
VS_RS12045 (VS_2556)	trmD	2746227..2746967 (-)	741	WP_009847608.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
VS_RS12050 (VS_2557)	rimM	2746994..2747548 (-)	555	WP_009847609.1	ribosome maturation factor RimM	-
VS_RS12055 (VS_2558)	rpsP	2747575..2747823 (-)	249	WP_004735508.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32510.72 Da
• Isoelectric Point: 7.6347

>NTDB_id=32288 VS_RS12020 WP_012604745.1 2742960..2743829(+) (pilD) [Vibrio atlanticus]
MEVFHYYPWLFPVLAFIFSLLIGSFLNVVIHRLPIMMEREWQQECSEYFSQYKIPAPEGKFNLSIPRSACPKCKTQLRIV
DNVPVLSWLFLKGKCHSCANPISARYPLVELLTAILCTVVASHFGFNYYAIALIFFTFALIAATFIDLDTMLLPDQITLP
LVWSGIALALFNISPVSLQDSVVGAMAGYLALWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQHLPMIILLSSLVGL
VFGLIQLRLKQQGIDKAFPFGPYLAIAGWVSLMWGNDIMGWYFTSVLGI

Nucleotide

• Length: 870 bp

>NTDB_id=32288 VS_RS12020 WP_012604745.1 2742960..2743829(+) (pilD) [Vibrio atlanticus]
ATGGAAGTATTTCACTACTATCCTTGGCTATTCCCCGTATTAGCTTTCATTTTTAGCCTTCTTATTGGCAGTTTCCTCAA
CGTAGTCATACACCGTTTACCGATTATGATGGAACGAGAGTGGCAACAAGAGTGCTCGGAGTATTTCTCTCAATATAAAA
TTCCAGCGCCAGAGGGGAAGTTCAATCTCAGTATCCCTCGTTCGGCTTGCCCGAAATGTAAAACCCAATTAAGAATCGTC
GACAATGTTCCAGTATTAAGCTGGTTGTTCTTGAAAGGTAAGTGTCATAGCTGTGCTAACCCAATCAGTGCTCGCTATCC
ATTGGTCGAACTGCTGACTGCAATACTATGCACCGTAGTGGCTAGCCACTTTGGTTTCAATTACTACGCCATTGCTTTGA
TTTTTTTCACCTTTGCATTGATTGCCGCAACCTTTATCGACCTAGATACCATGCTGTTACCTGATCAAATCACCTTACCT
TTGGTTTGGTCTGGTATCGCGTTAGCACTGTTTAACATCAGCCCAGTATCGCTTCAAGATTCCGTAGTTGGCGCAATGGC
CGGCTACCTAGCTTTGTGGTCTGTTTATTGGTTGTTCAAGCTACTAACAGGCAAAGAAGGCATGGGATATGGAGACTTCA
AACTCCTAGCCGCATTAGGTGCATGGCTTGGCTGGCAACACCTACCAATGATCATTCTTTTGTCGTCGCTGGTCGGTTTG
GTTTTCGGCTTGATTCAACTTCGCTTGAAACAGCAAGGCATAGATAAAGCGTTCCCATTTGGACCTTACCTGGCGATTGC
GGGTTGGGTAAGTTTGATGTGGGGCAATGACATCATGGGTTGGTATTTCACTTCTGTACTAGGGATTTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	74.048	100	0.74
pilD	Vibrio cholerae strain A1552	68.403	99.654	0.682
pilD	Acinetobacter nosocomialis M2	51.311	92.388	0.474
pilD	Acinetobacter baumannii D1279779	50.936	92.388	0.471
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436