Detailed information NTG0322824

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: Aba7835_RS14880
• Genome accession: NZ_CP033243
• Coordinates: 3063890..3065008 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347041.1
• Uniprot ID: A0A3R9E3W9
• Organism: Acinetobacter baumannii strain 7835
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3058890..3070008

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Aba7835_RS14870 (Aba7835_15075)	-	3062006..3062698 (-)	693	WP_001108516.1	YggS family pyridoxal phosphate-dependent enzyme	-
Aba7835_RS14875 (Aba7835_15080)	pilT	3062825..3063862 (+)	1038	WP_000355487.1	type IV pilus twitching motility protein PilT	Machinery gene
Aba7835_RS14880 (Aba7835_15085)	pilU	3063890..3065008 (+)	1119	WP_000347041.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
Aba7835_RS14885 (Aba7835_15090)	fur	3065078..3065515 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
Aba7835_RS14890 (Aba7835_15095)	-	3065627..3066025 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
Aba7835_RS14895 (Aba7835_15100)	-	3066080..3066403 (-)	324	WP_000442585.1	RnfH family protein	-
Aba7835_RS14900 (Aba7835_15105)	-	3066403..3067470 (-)	1068	WP_029642282.1	membrane protein	-
Aba7835_RS14905 (Aba7835_15115)	-	3067623..3068078 (-)	456	WP_000782976.1	bacteriohemerythrin	-
Aba7835_RS14910 (Aba7835_15120)	-	3068331..3069179 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41922.14 Da
• Isoelectric Point: 6.5789

>NTDB_id=322824 Aba7835_RS14880 WP_000347041.1 3063890..3065008(+) (pilU) [Acinetobacter baumannii strain 7835]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLQSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQYLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=322824 Aba7835_RS14880 WP_000347041.1 3063890..3065008(+) (pilU) [Acinetobacter baumannii strain 7835]
ATGGATTTTAATGACTTGCTCAATCTGATGGTTGAAAAAAAATCATCCGACCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACAGTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCTGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGTATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (29-270)

3D structure

• AlphaFold DB: A0A3R9E3W9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	68.194	99.731	0.68
pilU	Vibrio cholerae strain A1552	52.973	99.462	0.527
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392