Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EBA13_RS16575 Genome accession   NZ_CP033173
Coordinates   3496613..3498346 (-) Length   577 a.a.
NCBI ID   WP_024743351.1    Uniprot ID   -
Organism   Xanthomonas oryzae pv. oryzae strain CFBP8172     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3491613..3503346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EBA13_RS16555 (EBA13_16560) - 3492417..3494030 (+) 1614 WP_024743349.1 HAMP domain-containing sensor histidine kinase -
  EBA13_RS16560 (EBA13_16565) pilR 3494301..3495755 (+) 1455 WP_024743350.1 sigma-54 dependent transcriptional regulator Regulator
  EBA13_RS23080 - 3495902..3496159 (+) 258 WP_230078506.1 hypothetical protein -
  EBA13_RS16570 (EBA13_16575) - 3496152..3496604 (+) 453 WP_196381722.1 Fic family protein -
  EBA13_RS16575 (EBA13_16580) pilB 3496613..3498346 (-) 1734 WP_024743351.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EBA13_RS16580 (EBA13_16585) - 3498381..3499907 (-) 1527 WP_024743352.1 membrane protein -
  EBA13_RS16585 (EBA13_16590) - 3499877..3500452 (-) 576 WP_233488061.1 hypothetical protein -
  EBA13_RS16590 (EBA13_16595) - 3500523..3502112 (-) 1590 WP_024743354.1 phosphoethanolamine transferase -
  EBA13_RS16595 (EBA13_16600) pilA2 3502247..3502672 (-) 426 WP_024743355.1 pilin Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62872.97 Da        Isoelectric Point: 5.6097

>NTDB_id=321971 EBA13_RS16575 WP_024743351.1 3496613..3498346(-) (pilB) [Xanthomonas oryzae pv. oryzae strain CFBP8172]
MNSVSTTNLVGITGIARRLVQDGALEEAAARTAMEQAAAAKVPLPQWFAEKKLVSASQLAAANAVEFGMPLMDVSVFDAN
QNAVKLVSEELLQKYHVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASSASFGSSLG
NDDEEMGDLDVSTGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPEQVANMELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALMK
ASQGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=321971 EBA13_RS16575 WP_024743351.1 3496613..3498346(-) (pilB) [Xanthomonas oryzae pv. oryzae strain CFBP8172]
ATGAATTCAGTATCTACAACCAACCTCGTTGGCATCACCGGCATCGCTCGTCGCCTTGTGCAGGATGGCGCCCTGGAGGA
AGCCGCTGCGCGCACAGCGATGGAACAGGCGGCCGCTGCCAAGGTGCCGCTGCCGCAGTGGTTTGCCGAGAAGAAGCTGG
TGTCGGCATCGCAGCTGGCGGCGGCCAATGCAGTGGAATTCGGCATGCCACTGATGGATGTGTCTGTGTTCGACGCTAAC
CAGAACGCAGTCAAGCTAGTCAGCGAGGAGTTGCTCCAGAAGTACCATGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
GTTTGTGGGAGTGAGCAACCCGACTCAGACCCGTGCACTAGATGACATCAAGTTCCATACGAATCTGGTCGTCGAGCCGA
TTCTTGTCGATGAGGACCAAATTCGTCGCACATTGGAGCAATGGCAGGCAAGCAGCGCCTCATTTGGATCATCGCTTGGC
AACGATGATGAGGAAATGGGAGATCTGGACGTCTCCACCGGCGACGAAGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAAGTGCTGGTGGATGCGATCCGGCGGGGGGCCTCGGACA
TCCATTTCGAGCCGTACGAAGACGACTACCGGGTGCGGTTGCGGATCGACGGGTTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAGTCAGCGCATCGCGGCGCGTTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTACCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGATTTCCGTGTCAGTACTTTGCCGACCCTGTTCGGTGAGA
AGGTGGTGCTGCGTATTCTGGACGGTAGCGCGGCCAAGCTGGGCATCGAGAAGCTAGGCTACGAGGCGGACCAGCAAAAG
CTGTTCCTGGATGCAATTCATAAGCCTTACGGGATGGTGCTGGTGACCGGGCCCACCGGTTCGGGCAAGACGGTGTCGCT
GTATACGGCGCTGGGCATCCTCAACGACGAAACTCGGAATATCTCGACGGCCGAGGATCCGGTCGAAATTCGCCTGCCTG
GCGTCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCGCTGCGTTCGTTCCTGCGACAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCCACGCTGCATACCAACGATGCGCCACAGACCATCGCGCGTCTGATGAACATGGGGATCGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAATAACTGCAAGCGCAAGTCGACGTTGCCG
GAGCATGCACTGCTGGCGGAAGGCTTTACGCCGGAGCAGGTCGCGAATATGGAGCTTTATGAAGCCGTGGGTTGCGACGA
GTGCACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATTGGCGCGATCGTGCTGG
AAGGCGGCAATGCTATGCAGATTGCCGAGGCCGCTCAGAAGATCGGCATCCGGGACTTGCGGCAGTCGGCGTTGATGAAG
GCCTCGCAAGGCGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.44

0.556
  pilB Acinetobacter baylyi ADP1

56.338

98.44

0.555
  pilB Legionella pneumophila strain ERS1305867

52.289

98.44

0.515
  pilB Vibrio cholerae strain A1552

49.74

100

0.497
  pilF Neisseria gonorrhoeae MS11

49.648

98.44

0.489
  pilB Vibrio campbellii strain DS40M4

46.087

99.653

0.459
  pilB Vibrio parahaemolyticus RIMD 2210633

46.004

97.574

0.449
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.394

91.508

0.36

Multiple sequence alignment