Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   D0812_RS01740 Genome accession   NZ_CP033137
Coordinates   350921..352669 (+) Length   582 a.a.
NCBI ID   WP_045412013.1    Uniprot ID   -
Organism   Vibrio owensii strain 1700302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 345921..357669
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D0812_RS01720 (D0812_01720) pilM 348217..349233 (+) 1017 WP_054824717.1 type IV pilus assembly protein PilM Machinery gene
  D0812_RS01725 (D0812_01725) pilN 349217..349795 (+) 579 WP_054824716.1 PilN domain-containing protein Machinery gene
  D0812_RS01730 (D0812_01730) pilO 349788..350381 (+) 594 WP_009705519.1 type 4a pilus biogenesis protein PilO Machinery gene
  D0812_RS01735 (D0812_01735) pilP 350371..350886 (+) 516 WP_005438430.1 pilus assembly protein PilP Machinery gene
  D0812_RS01740 (D0812_01740) pilQ 350921..352669 (+) 1749 WP_045412013.1 type IV pilus secretin PilQ family protein Machinery gene
  D0812_RS01745 (D0812_01745) aroK 352859..353377 (+) 519 WP_005438434.1 shikimate kinase AroK -
  D0812_RS01750 (D0812_01750) aroB 353409..354509 (+) 1101 WP_054824715.1 3-dehydroquinate synthase -
  D0812_RS01755 (D0812_01755) - 354563..356056 (+) 1494 WP_054824714.1 AAA family ATPase -
  D0812_RS01760 (D0812_01760) - 356126..356965 (+) 840 WP_005438442.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 582 a.a.        Molecular weight: 63877.96 Da        Isoelectric Point: 4.5124

>NTDB_id=321728 D0812_RS01740 WP_045412013.1 350921..352669(+) (pilQ) [Vibrio owensii strain 1700302]
MKMRQGLTLTPRKLAGIFSLWFFLLLAPFVAAQEVKSNSLKTIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESIEIFRDTSTTRLVAYIDEEYTHDYRLTGRYLEIKVGKIKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGDGEGEDGGSSVGIDDFLNVNLAATSPNA
TSLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQ
ITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFR
RSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1749 bp        

>NTDB_id=321728 D0812_RS01740 WP_045412013.1 350921..352669(+) (pilQ) [Vibrio owensii strain 1700302]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAGCAGGGATCTTCTCTTTGTGGTTCTTTTTACTTTTAGC
CCCTTTTGTAGCAGCACAGGAAGTAAAGTCTAACTCGCTAAAGACCATCGACTTTCGTACAAACAAAGACAAAGATGCCG
TGATTGTTGTAGAACTTGCGTCCCCTGCTGCAATCGTTGATATCAAGCGCGTACAGGAAGGGTTGAGTATCGACTTACTC
AACACATCTGTAAAAGACGACCAGCTTTACCTTCTGGATGTAAAAGACTTTTCTACGGTCGTCGAGAGTATTGAAATATT
CCGAGATACCTCAACCACACGTTTAGTTGCGTATATTGATGAGGAATACACTCACGATTACCGACTGACAGGGCGTTACC
TAGAGATCAAAGTCGGTAAGATAAAAGCCAACGAAAAAGCACCGGATAAGAGCATTCTAGAAAAAGAAGGGAAGCTGATT
TCAATTAACTTCCAAGACATCCCAGTGCGAAACGTATTGCAACTCATCGCCGACTACAACCAATTTAACTTAGTGGTATC
TGACTCCGTTGAAGGCAACTTAACTTTGCGTCTTGATGGTGTGCCTTGGCAGCAAGTGCTCGATATCATTCTTCAGGTAA
AAGGCTTGGATAAGCGTGTTGATGGCAACGTGATCTTGGTGGCGCCGAAAGACGAACTGGATCTGCGTGAAAAGCAACAA
TTAGAGAAACAGAAACTTGAGCAGGAGATGGGGGAACTTTCTTCTGAGATCATCAAAGTTAACTTTGCGAAAGCGTCGGA
CATTGCCGAGATGATTAATGGCGAGGGGAATATCAGTATGCTCTCAGAACGTGGCAGCATGACCATTGATGAGCGGACGA
ACTCACTATTAATTAGAGAACTACCAGAGAACATTGAGGTTATCCGAGAGATCATTGAGTCTTTGGATATTCCGGTCAAA
CAAGTTCAGATTGAAGCGCGAATCGTCACCATCAATGAAGGTAATATGGACGAACTTGGTGTGCGCTGGGGCTTCACCTC
TATTAACGGCAGCCACACTGTCGGTGGTTCGATTGAGAATAACCTTGCGACAATTGGCCTGTACGATGGCGGCGGTGATG
GCGAAGGTGAAGATGGTGGCAGCAGTGTTGGTATTGATGACTTCCTAAACGTGAATTTGGCTGCAACCAGTCCGAATGCG
ACCAGTCTTGCTTTCCAAGTTGCGAAGTTAGGCTCAGACACACTGCTTGATTTAGAGCTTTCTGCGCTGCAACAAGAATC
CAAGGCAGAGATTATTTCGAGCCCGCGATTGATTACCACTAACAAGAAGCCTGCTTATATCGAGCAAGGTACCGAAATCC
CGTACCTTGAGTCTTCTTCCAGTGGCGCAACGTCTGTCACGTTTAAAAAAGCCGTATTGAGCTTAAAAGTGACCCCACAA
ATTACTCCTGACAATCGTTTAGTGTTGGATTTGAGCGTTACGCAAGATAGACCTGGTCAAGTTGTAAAAACAGGCACAGG
TGAAGCGGTCGCGATCGATACTCAACGCATTGGCACACAAGTCTTAGTCAATAACGGTGAAACGGTTGTGTTGGGTGGTA
TTTTTCAACATAGCATCACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTTCGT
CGAAGTTATGAAAATGTCGGCAAAAGTGAACTGTTGATTTTTGTCACGCCTAAAGTTGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(422-580)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

95.876

100

0.959
  pilQ Vibrio cholerae strain A1552

76.761

97.595

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

76.761

97.595

0.749
  pilQ Pseudomonas aeruginosa PAK

40.522

98.797

0.4
  pilQ Legionella pneumophila strain ERS1305867

40.998

96.392

0.395
  pilQ Legionella pneumophila strain Lp02

40.998

96.392

0.395
  pilQ Acinetobacter baumannii D1279779

37.018

100

0.38
  pilQ Acinetobacter baumannii strain A118

37.018

100

0.38
  comQ Acinetobacter baylyi ADP1

37.177

99.828

0.371

Multiple sequence alignment