Detailed information NTG0321503

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: EAV54_RS14325
• Genome accession: NZ_CP033110
• Coordinates: 2979355..2980473 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347034.1
• Uniprot ID: A0A009HMD8
• Organism: Acinetobacter baumannii ATCC 17978
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2974355..2985473

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EAV54_RS14315 (EAV54_14370)	-	2977471..2978163 (-)	693	WP_001108509.1	YggS family pyridoxal phosphate-dependent enzyme	-
EAV54_RS14320 (EAV54_14375)	pilT	2978290..2979327 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
EAV54_RS14325 (EAV54_14380)	pilU	2979355..2980473 (+)	1119	WP_000347034.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
EAV54_RS14330 (EAV54_14385)	fur	2980543..2980980 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
EAV54_RS14335 (EAV54_14390)	-	2981092..2981490 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
EAV54_RS14340 (EAV54_14395)	-	2981545..2981868 (-)	324	WP_000442590.1	RnfH family protein	-
EAV54_RS14345 (EAV54_14400)	-	2981868..2982935 (-)	1068	WP_004748097.1	hypothetical protein	-
EAV54_RS14350 (EAV54_14410)	-	2983088..2983543 (-)	456	WP_000782976.1	bacteriohemerythrin	-
EAV54_RS14355 (EAV54_14415)	-	2983796..2984644 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41891.09 Da
• Isoelectric Point: 6.6943

>NTDB_id=321503 EAV54_RS14325 WP_000347034.1 2979355..2980473(+) (pilU) [Acinetobacter baumannii ATCC 17978]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFADTRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=321503 EAV54_RS14325 WP_000347034.1 2979355..2980473(+) (pilU) [Acinetobacter baumannii ATCC 17978]
ATGGATTTTAATGACCTACTCAACCTGATGGTTGAAAAGAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGCCTTTCTGGGGAAGTAATTGGTCAACTGTTACATTCCA
TTATGAGTGAAAAACAACGCAAAGAATTTGCAGACACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGTGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGATCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAGGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (34-270)

3D structure

• AlphaFold DB: A0A009HMD8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.661	100	0.769
pilU	Pseudomonas stutzeri DSM 10701	69.022	98.925	0.683
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.908	93.011	0.39