Detailed information NTG0321477

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: EAV54_RS01515
• Genome accession: NZ_CP033110
• Coordinates: 318115..319173 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii ATCC 17978
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 313115..324173

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EAV54_RS01500 (EAV54_01520)	-	314220..314552 (+)	333	WP_000169602.1	hypothetical protein	-
EAV54_RS01505 (EAV54_01525)	-	314555..315379 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
EAV54_RS01510 (EAV54_01530)	ponA	315397..317952 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
EAV54_RS01515 (EAV54_01535)	pilM	318115..319173 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
EAV54_RS01520 (EAV54_01540)	pilN	319173..319814 (+)	642	WP_000201223.1	PilN domain-containing protein	Machinery gene
EAV54_RS01525 (EAV54_01545)	pilO	319811..320551 (+)	741	WP_000076102.1	type 4a pilus biogenesis protein PilO	Machinery gene
EAV54_RS01530 (EAV54_01550)	pilP	320562..321089 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
EAV54_RS01535 (EAV54_01555)	pilQ	321157..323322 (+)	2166	WP_001017031.1	type IV pilus secretin PilQ	Machinery gene
EAV54_RS01540 (EAV54_01560)	aroK	323334..323903 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=321477 EAV54_RS01515 WP_002014066.1 318115..319173(+) (pilM) [Acinetobacter baumannii ATCC 17978]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=321477 EAV54_RS01515 WP_002014066.1 318115..319173(+) (pilM) [Acinetobacter baumannii ATCC 17978]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGACGCGGTAGT
TCAGCAGGCGGCGCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCATTCTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423