Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   EAW52_RS17450 Genome accession   NZ_CP033105
Coordinates   3730922..3732262 (+) Length   446 a.a.
NCBI ID   WP_122043377.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3725922..3737262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS17425 (EAW52_17425) pgeF 3726017..3726760 (-) 744 WP_122043373.1 peptidoglycan editing factor PgeF -
  EAW52_RS17430 (EAW52_17430) rluD 3726757..3727719 (-) 963 WP_122044843.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EAW52_RS17435 (EAW52_17435) - 3727877..3728890 (+) 1014 WP_122043374.1 outer membrane protein assembly factor BamD -
  EAW52_RS26435 (EAW52_17440) - 3729107..3729346 (+) 240 WP_122043375.1 PP0621 family protein -
  EAW52_RS17445 (EAW52_17445) pilS 3729336..3730919 (+) 1584 WP_122043376.1 PAS domain-containing sensor histidine kinase Regulator
  EAW52_RS17450 (EAW52_17450) pilR 3730922..3732262 (+) 1341 WP_122043377.1 sigma-54 dependent transcriptional regulator Regulator
  EAW52_RS17455 (EAW52_17455) thiO 3732263..3733372 (-) 1110 WP_122043378.1 glycine oxidase ThiO -
  EAW52_RS26885 (EAW52_17460) - 3733480..3733977 (+) 498 WP_162997655.1 GspH/FimT family pseudopilin -
  EAW52_RS17465 (EAW52_17465) - 3734210..3734743 (+) 534 WP_122044845.1 GspH/FimT family pseudopilin -
  EAW52_RS17470 (EAW52_17470) pilV 3734752..3735300 (+) 549 WP_122043379.1 type IV pilus modification protein PilV -
  EAW52_RS17475 (EAW52_17475) - 3735266..3736042 (+) 777 WP_122043380.1 PilW family protein -
  EAW52_RS17480 (EAW52_17480) - 3736039..3736602 (+) 564 WP_122043381.1 PilX N-terminal domain-containing pilus assembly protein -

Sequence

Protein


Download         Length: 446 a.a.        Molecular weight: 49798.27 Da        Isoelectric Point: 6.2054

>NTDB_id=321419 EAW52_RS17450 WP_122043377.1 3730922..3732262(+) (pilR) [Pseudomonas sp. LTJR-52]
MNRQKVLIVDDEPDIRELLEITLGRMKLDTFSAKNVKDAKDLLARESFDLCLTDMRLPDGSGLDLVQYIQQKYPNLPVAM
ITAFGSLDTAIQALKAGAFDYLTKPVDLPRLRELVSTALRLSSSAPTPIKVADHRLLGDSPPMRTLRAQIGKLARSQAPV
YISGESGSGKELVARLIHEQGPRADRPFIPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAAQGGTLFLDEVADL
PLAMQVKLLRAIQEKAVRPVGGQQEVALDVRILSATHKDLAAEVAADRFRQDLFYRLNVIELRVPPLRERREDIAELCST
ILKRLAKESQQDLAQLTDDALAKLKSYRFPGNVRELENMLERAYTLCEDNLIKPSDLRLADTPQSSSAGETSLANISNLE
DYLEEIERKLIMQALEETRWNRTAAAERLGLSFRSMRYRLKKLGID

Nucleotide


Download         Length: 1341 bp        

>NTDB_id=321419 EAW52_RS17450 WP_122043377.1 3730922..3732262(+) (pilR) [Pseudomonas sp. LTJR-52]
ATGAATCGACAGAAAGTCCTGATCGTTGATGACGAACCCGATATCCGAGAGCTGTTAGAGATCACATTGGGTCGCATGAA
GCTCGACACCTTTAGCGCAAAGAACGTAAAAGACGCAAAGGATCTGTTGGCTCGGGAATCCTTTGACTTGTGTCTGACCG
ACATGCGCCTTCCAGATGGCTCCGGTCTTGATCTCGTGCAATACATCCAGCAGAAATATCCAAACCTTCCCGTAGCCATG
ATTACCGCTTTTGGAAGTCTGGATACGGCCATCCAGGCATTGAAAGCCGGCGCCTTCGATTACCTGACCAAACCTGTTGA
TCTTCCTCGGCTCCGGGAGCTTGTAAGCACCGCCTTACGCCTGAGCAGCTCAGCGCCTACCCCTATCAAAGTGGCAGATC
ACAGGCTGCTGGGTGACTCACCGCCCATGCGTACGCTACGGGCTCAGATCGGCAAACTCGCTCGCAGCCAGGCGCCCGTC
TATATCAGCGGTGAGTCCGGCAGCGGTAAAGAGTTGGTCGCCCGACTGATTCACGAGCAAGGCCCTCGTGCGGATCGCCC
CTTTATTCCGGTCAACTGTGGCGCCATTCCATCAGAGCTGATGGAAAGCGAGTTTTTTGGTCACAAAAAAGGCAGCTTTA
CGGGTGCCATTGAAGACAAGCAGGGCCTATTCCAGGCCGCCCAGGGCGGTACTTTATTTCTGGATGAGGTCGCCGATCTG
CCGCTAGCCATGCAGGTAAAACTGCTTCGTGCCATTCAGGAAAAGGCTGTACGCCCCGTCGGTGGGCAACAGGAAGTAGC
GCTGGATGTACGGATACTAAGTGCCACACATAAAGATCTGGCCGCCGAAGTAGCCGCTGATCGCTTCCGGCAAGACTTAT
TTTATCGCCTCAACGTCATTGAGCTTCGCGTACCCCCGCTACGTGAACGACGCGAAGACATTGCGGAACTGTGCTCGACT
ATCCTGAAACGTCTCGCCAAGGAATCACAACAGGATCTGGCCCAGCTGACAGATGACGCTCTCGCCAAACTCAAGAGCTA
TCGCTTTCCGGGTAATGTTCGCGAGTTGGAGAACATGCTTGAGCGAGCCTATACCCTTTGTGAAGACAACCTGATCAAAC
CAAGCGATTTGCGCCTTGCAGATACTCCTCAGTCCTCCAGCGCTGGCGAAACCAGTCTGGCCAACATCAGTAATCTTGAG
GATTACCTGGAAGAGATCGAGCGAAAGCTGATCATGCAAGCGCTTGAGGAGACGCGCTGGAACCGTACAGCCGCCGCTGA
ACGGCTCGGGCTTTCCTTCCGCTCGATGCGGTATCGCCTGAAAAAGTTGGGCATCGATTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(137-302)

Bacterial regulatory protein, Fis family (HTH_8)

(403-443)

Response regulator receiver domain (Response_reg)

(6-115)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

83.184

100

0.832
  pilR Acinetobacter baumannii strain A118

50.98

100

0.525
  luxO Vibrio cholerae strain A1552

37.33

99.103

0.37

Multiple sequence alignment