Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   EAW52_RS17445 Genome accession   NZ_CP033105
Coordinates   3729336..3730919 (+) Length   527 a.a.
NCBI ID   WP_122043376.1    Uniprot ID   -
Organism   Pseudomonas sp. LTJR-52     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3724336..3735919
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EAW52_RS17425 (EAW52_17425) pgeF 3726017..3726760 (-) 744 WP_122043373.1 peptidoglycan editing factor PgeF -
  EAW52_RS17430 (EAW52_17430) rluD 3726757..3727719 (-) 963 WP_122044843.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EAW52_RS17435 (EAW52_17435) - 3727877..3728890 (+) 1014 WP_122043374.1 outer membrane protein assembly factor BamD -
  EAW52_RS26435 (EAW52_17440) - 3729107..3729346 (+) 240 WP_122043375.1 PP0621 family protein -
  EAW52_RS17445 (EAW52_17445) pilS 3729336..3730919 (+) 1584 WP_122043376.1 PAS domain-containing sensor histidine kinase Regulator
  EAW52_RS17450 (EAW52_17450) pilR 3730922..3732262 (+) 1341 WP_122043377.1 sigma-54 dependent transcriptional regulator Regulator
  EAW52_RS17455 (EAW52_17455) thiO 3732263..3733372 (-) 1110 WP_122043378.1 glycine oxidase ThiO -
  EAW52_RS26885 (EAW52_17460) - 3733480..3733977 (+) 498 WP_162997655.1 GspH/FimT family pseudopilin -
  EAW52_RS17465 (EAW52_17465) - 3734210..3734743 (+) 534 WP_122044845.1 GspH/FimT family pseudopilin -
  EAW52_RS17470 (EAW52_17470) pilV 3734752..3735300 (+) 549 WP_122043379.1 type IV pilus modification protein PilV -

Sequence

Protein


Download         Length: 527 a.a.        Molecular weight: 58997.02 Da        Isoelectric Point: 6.9686

>NTDB_id=321418 EAW52_RS17445 WP_122043376.1 3729336..3730919(+) (pilS) [Pseudomonas sp. LTJR-52]
MSADFFHYNLQDSRILRLYHYYRVIVGLVFILLVSTNLDDNLLKVTEGKNFVVGSWIYLAGNILAIFLPPLRKQKQIFAL
ALVDIGLLTYLMYSAGGTPSGIGNLIIITVSIANILLVGRIGILLAALATLALIYATAYRTLSLPAEANQYVQAGGLGAL
CFAAAFLVQSLVSRLSKSQTLAEERAHVVADLENLNALILDRMRTGILVLDSRMAPILANPAARELLGHPDLDTEDLNDQ
CPELMDRLLQWERNRTLRPAPMQAFSEGPLIQPSFVPIQRSGNQHTLIFLENISRIAQQAQQLKLASLGRLTAGIAHEIR
NPLGAISHAAQLLQESEVLDQHDLRLAQIIQDHSRRMNIIIENVLQLSRRRQAEPQLLDLKYWVHRFAGEFRTTLREQQF
VYVQTTSGTIQTRMDPSQLTQVLTNLVQNGLRHSSKLHGKGQVWLDLFRDPDSDLPILDVIDDGPGVPEDQITNLFEPFF
TTESKGTGLGLYISRELCESNQARLDYRQQEQGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1584 bp        

>NTDB_id=321418 EAW52_RS17445 WP_122043376.1 3729336..3730919(+) (pilS) [Pseudomonas sp. LTJR-52]
GTGAGCGCTGATTTTTTTCATTACAACCTGCAGGACAGTCGTATCCTGAGGTTGTATCACTACTACCGGGTCATTGTCGG
GCTGGTTTTTATCCTTCTGGTTTCCACCAATCTGGACGACAACCTTCTGAAGGTAACCGAGGGTAAAAACTTTGTGGTCG
GCAGCTGGATTTACCTAGCGGGTAATATCCTGGCGATTTTTCTGCCACCACTGCGCAAGCAAAAACAGATCTTTGCCCTG
GCTCTAGTGGATATCGGGCTGCTGACCTATCTCATGTACAGTGCTGGCGGTACACCGAGTGGTATTGGCAACCTCATCAT
CATTACCGTATCGATCGCCAACATTCTTCTCGTTGGGCGTATTGGAATTCTGCTGGCGGCCTTGGCTACCCTTGCCCTTA
TTTATGCAACCGCCTATAGAACGCTTAGCCTTCCCGCAGAGGCCAATCAGTATGTTCAGGCGGGAGGCCTGGGCGCGCTG
TGCTTTGCCGCCGCATTTCTGGTGCAAAGTCTCGTCAGCCGATTGTCCAAAAGCCAGACCCTGGCAGAAGAGCGGGCGCA
CGTCGTTGCCGATCTGGAAAATCTGAATGCGCTGATCCTCGATCGTATGCGCACCGGCATTCTGGTTCTGGACTCAAGAA
TGGCGCCTATCCTGGCCAATCCTGCCGCTCGAGAGCTGCTCGGTCATCCAGACCTGGATACCGAGGATCTGAATGATCAA
TGCCCGGAATTGATGGACCGGCTACTACAGTGGGAAAGGAATCGGACGCTTCGCCCTGCACCCATGCAGGCGTTTAGCGA
AGGCCCACTGATCCAGCCCAGCTTCGTCCCTATTCAGCGGAGCGGCAATCAGCATACTCTGATTTTTTTGGAAAACATCT
CTCGTATTGCTCAGCAGGCACAGCAGCTGAAGCTTGCATCACTGGGACGCCTCACGGCGGGCATCGCCCATGAAATTCGC
AATCCTCTAGGAGCCATAAGCCACGCGGCACAGCTTCTTCAGGAATCAGAAGTGCTCGATCAGCATGATCTTCGCCTGGC
CCAGATCATTCAGGATCACTCCCGGCGCATGAACATCATCATCGAGAATGTCCTGCAACTTTCTAGACGCCGCCAGGCAG
AGCCCCAGCTGCTGGACCTCAAATACTGGGTGCACCGCTTTGCAGGTGAATTCAGAACCACTCTACGTGAGCAACAGTTT
GTCTATGTGCAAACGACATCGGGTACGATACAGACCCGCATGGACCCTAGCCAGCTGACTCAGGTGCTAACCAACCTCGT
TCAAAATGGCCTACGCCATAGCAGCAAGCTGCACGGCAAGGGACAAGTGTGGCTGGATCTCTTCCGTGACCCCGACTCAG
ACCTTCCCATATTGGATGTCATTGATGATGGGCCAGGTGTACCTGAAGACCAGATCACCAACCTTTTCGAACCCTTCTTT
ACCACCGAAAGCAAAGGAACCGGGCTCGGCCTCTATATTTCCCGCGAATTATGCGAGAGTAATCAGGCCCGCCTCGACTA
CCGCCAGCAGGAACAAGGCGGCTGCTTCCGCATTACCTTTGCCCATCCGCGCAAGTTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(307-371)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(415-520)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

64.162

98.482

0.632

Multiple sequence alignment