Detailed information NTG0319855

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: D9N00_RS03640
• Genome accession: NZ_CP032871
• Coordinates: 629955..631019 (+)
• Length: 354 a.a.
• NCBI ID: WP_003378852.1
• Uniprot ID: A0A2G9KZJ9
• Organism: Pseudomonas syringae pv. actinidiae strain P220
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 624955..636019

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D9N00_RS03630 (D9N00_03630)	-	625710..626978 (+)	1269	WP_003378855.1	malic enzyme-like NAD(P)-binding protein	-
D9N00_RS03635 (D9N00_03635)	-	627304..629748 (-)	2445	WP_017682643.1	penicillin-binding protein 1A	-
D9N00_RS03640 (D9N00_03640)	pilM	629955..631019 (+)	1065	WP_003378852.1	pilus assembly protein PilM	Machinery gene
D9N00_RS03645 (D9N00_03645)	-	631019..631645 (+)	627	WP_003378851.1	PilN domain-containing protein	-
D9N00_RS03650 (D9N00_03650)	pilO	631642..632265 (+)	624	WP_003378850.1	type 4a pilus biogenesis protein PilO	-
D9N00_RS03655 (D9N00_03655)	pilP	632262..632789 (+)	528	WP_003378849.1	type 4a pilus biogenesis lipoprotein PilP	-
D9N00_RS03660 (D9N00_03660)	pilQ	632837..634942 (+)	2106	WP_017682642.1	type IV pilus secretin PilQ	Machinery gene
D9N00_RS03665 (D9N00_03665)	aroK	634947..635465 (+)	519	WP_003378847.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38103.62 Da
• Isoelectric Point: 4.7008

>NTDB_id=319855 D9N00_RS03640 WP_003378852.1 629955..631019(+) (pilM) [Pseudomonas syringae pv. actinidiae strain P220]
MFELFGKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTNVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=319855 D9N00_RS03640 WP_003378852.1 629955..631019(+) (pilM) [Pseudomonas syringae pv. actinidiae strain P220]
GTGTTCGAACTCTTTGGCAAGAAGGCCAATACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACTCGTTACAAGGTCGAGTCTTACGCAGTAGAGCCGTTACCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGTCAGGCGTTGTCTCGCGTACTGGTCAAGGCCAAGACCAACGTCAAGATTGTGGCGGTT
GCCGTGGCGGGTTCCGCTGTGATCACCAAAACCATCGAGATGGACGCCGGTCTCTCCGACGACGACATGGAAAATCAGCT
TAAGCTTGAAGCTGATCAGTACATCCCTTATCCGCTCGAAGAAGTTGCCATCGATTTTGAAGTGCAGGGCTACTCGGTCC
GCAACCCGGAACGTGTCGAAGTCCTGTTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTGCGCGAGGCTGCGCTGGCTCTC
GCCGGGCTGACGGCGCGTGTCGTGGACGTCGAGGCCTACGCGCTGGAGCGTTCATTCGGGCTTCTGGCTGCGCAACTGGG
CAACGGTCATGACGAACTGACCGTTGCCGTGGTAGACATCGGCGCGACCATGACCACCCTCAGCGTGCTGCACCACGGCC
GAATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGCCAGCTGACTGACGAAATTCAGCGTCGCTACGGGCTTTCCATG
GAAGAGGCCGGGCTGGCCAAGAAGCAGGGCGGGCTGCCTGACGATTATGTCAGCGAAGTGCTGGACCCCTTCAAGGACGC
GCTCGTGCAACAGGTTTCCCGATCCCTGCAGTTCTTCTTTGCGGCCGGTCAGTACAACTCCGTGGATCACATCATGCTGG
CAGGCGGTACGGCTTCGATTTCAGGGCTGGAGCATTTGATTCAGCGACGTATCGGCACGCCGACCATGGTTGCCAACCCG
TTTGCCGACATGGCCTTGAGCTCCAAGGTCAATGCCGGTGCGCTGGCCAGCGATGCGCCTGCCCTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A2G9KZJ9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.761	100	0.469