Detailed information NTG0318460

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: D7A46_RS14520
• Genome accession: NZ_CP032743
• Coordinates: 2861068..2862126 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain C25
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2856068..2867126

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D7A46_RS14505 (D7A46_14495)	-	2857173..2857505 (+)	333	WP_000169594.1	hypothetical protein	-
D7A46_RS14510 (D7A46_14500)	-	2857508..2858332 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
D7A46_RS14515 (D7A46_14505)	ponA	2858350..2860905 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
D7A46_RS14520 (D7A46_14510)	pilM	2861068..2862126 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
D7A46_RS14525 (D7A46_14515)	pilN	2862126..2862767 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
D7A46_RS14530 (D7A46_14520)	pilO	2862764..2863504 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
D7A46_RS14535 (D7A46_14525)	pilP	2863515..2864042 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
D7A46_RS14540 (D7A46_14530)	pilQ	2864110..2866275 (+)	2166	WP_001017033.1	type IV pilus secretin PilQ family protein	Machinery gene
D7A46_RS14545 (D7A46_14535)	aroK	2866287..2866856 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=318460 D7A46_RS14520 WP_002014066.1 2861068..2862126(+) (pilM) [Acinetobacter baumannii strain C25]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=318460 D7A46_RS14520 WP_002014066.1 2861068..2862126(+) (pilM) [Acinetobacter baumannii strain C25]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423