Detailed information NTG0318442

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D7A46_RS08330
• Genome accession: NZ_CP032743
• Coordinates: 1575306..1576424 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347039.1
• Uniprot ID: A0A9P2XLL8
• Organism: Acinetobacter baumannii strain C25
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1570306..1581424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D7A46_RS08320 (D7A46_08315)	-	1573422..1574114 (-)	693	WP_001108512.1	YggS family pyridoxal phosphate-dependent enzyme	-
D7A46_RS08325 (D7A46_08320)	pilT	1574241..1575278 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
D7A46_RS08330 (D7A46_08325)	pilU	1575306..1576424 (+)	1119	WP_000347039.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D7A46_RS08335 (D7A46_08330)	fur	1576494..1576931 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
D7A46_RS08340 (D7A46_08335)	-	1577043..1577441 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
D7A46_RS08345 (D7A46_08340)	-	1577496..1577819 (-)	324	WP_000442587.1	RnfH family protein	-
D7A46_RS08350 (D7A46_08345)	-	1577819..1578886 (-)	1068	WP_002019572.1	hypothetical protein	-
D7A46_RS08360 (D7A46_08355)	-	1579039..1579494 (-)	456	WP_000782976.1	bacteriohemerythrin	-
D7A46_RS08365 (D7A46_08360)	-	1579747..1580595 (-)	849	WP_000336548.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41933.13 Da
• Isoelectric Point: 6.6944

>NTDB_id=318442 D7A46_RS08330 WP_000347039.1 1575306..1576424(+) (pilU) [Acinetobacter baumannii strain C25]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRREFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=318442 D7A46_RS08330 WP_000347039.1 1575306..1576424(+) (pilU) [Acinetobacter baumannii strain C25]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCTTCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAACAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAGAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTTGTAGGCGCGACAG
GCACAGGTAAATCTACTTCACTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAAAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.565	98.925	0.688
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.486	93.011	0.395