Detailed information NTG0317870

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: E8M05_RS10690
• Genome accession: NZ_CP039457
• Coordinates: 2049763..2050761 (-)
• Length: 332 a.a.
• NCBI ID: WP_003066863.1
• Uniprot ID: E0PGS8
• Organism: Streptococcus pasteurianus strain WUSP067
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2044763..2055761

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E8M05_RS10670 (E8M05_10660)	treP	2045292..2047265 (-)	1974	WP_048790987.1	PTS system trehalose-specific EIIBC component	-
E8M05_RS10675 (E8M05_10665)	treR	2047462..2048175 (+)	714	WP_003066857.1	trehalose operon repressor	Regulator
E8M05_RS10680 (E8M05_10670)	-	2048585..2048935 (-)	351	Protein_2008	isopeptide-forming domain-containing fimbrial protein	-
E8M05_RS10690 (E8M05_10680)	ruvB	2049763..2050761 (-)	999	WP_003066863.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
E8M05_RS10695 (E8M05_10685)	nrdG	2050890..2051504 (-)	615	WP_003066866.1	anaerobic ribonucleoside-triphosphate reductase activating protein	-
E8M05_RS10700 (E8M05_10690)	-	2051545..2052051 (-)	507	WP_003066869.1	GNAT family N-acetyltransferase	-
E8M05_RS11255	-	2052063..2052224 (-)	162	WP_003066871.1	hypothetical protein	-
E8M05_RS10705 (E8M05_10695)	nrdD	2052450..2054666 (-)	2217	WP_003066874.1	anaerobic ribonucleoside-triphosphate reductase	-

Sequence

Protein

• Length: 332 a.a.
• Molecular weight: 37509.92 Da
• Isoelectric Point: 4.5455

>NTDB_id=317870 E8M05_RS10690 WP_003066863.1 2049763..2050761(-) (ruvB) [Streptococcus pasteurianus strain WUSP067]
MSRILDNDIMGDEEFAERTLRPQFLKEYIGQDKVKNQLKIFIEAAKLRDESLDHVLLFGPPGLGKTTMAFVITNELGVNL
KQTSGPAIEKAGDLVAILNDLEPGDVLFIDEIHRMPMAVEEVLYSAMEDFYIDIMIGNGETSRSVHLDLPPFTLIGATTR
AGMLSNPLRARFGITGHMEYYDVEDLTEIVERTADIFEMEIVHEAAQELARRSRGTPRIANRLLKRVRDYAQIMGDGIIT
KDITDKALTMLDVDHEGLDYIDQKILRTMIEVYNGGPVGLGTLSVNIAEERDTVEDMYEPYLIQKGFIMRTRTGRVATEK
AYEHLGYPYSEK

Nucleotide

• Length: 999 bp

>NTDB_id=317870 E8M05_RS10690 WP_003066863.1 2049763..2050761(-) (ruvB) [Streptococcus pasteurianus strain WUSP067]
ATGAGCAGAATTTTAGATAATGATATTATGGGTGATGAGGAATTTGCGGAGCGCACGCTTCGTCCTCAGTTTTTAAAAGA
ATATATCGGACAAGATAAGGTTAAAAATCAGCTAAAAATCTTTATTGAGGCAGCAAAATTACGTGACGAGTCTTTGGACC
ATGTGCTATTGTTTGGACCTCCAGGTCTTGGTAAAACGACAATGGCATTTGTGATTACTAATGAATTAGGGGTGAATTTA
AAACAGACATCTGGACCTGCTATCGAAAAAGCTGGTGATTTGGTTGCTATCTTGAATGATTTGGAACCTGGTGATGTGCT
TTTTATTGACGAAATTCATCGTATGCCAATGGCTGTGGAAGAAGTACTTTATAGCGCCATGGAAGATTTTTATATTGACA
TCATGATTGGCAATGGCGAGACTAGTCGTAGTGTTCACTTGGATTTACCGCCGTTTACCCTGATTGGTGCGACAACACGT
GCTGGTATGCTTTCTAATCCTTTACGTGCTCGTTTTGGCATTACTGGGCATATGGAATACTATGACGTTGAAGATTTGAC
AGAAATTGTTGAACGCACGGCAGATATTTTTGAAATGGAAATTGTCCATGAAGCTGCCCAAGAATTGGCACGACGTAGTC
GTGGAACACCTCGTATTGCTAATCGTCTCTTAAAACGTGTACGTGATTACGCCCAAATTATGGGAGATGGTATTATCACA
AAAGATATTACCGATAAAGCATTGACGATGTTAGATGTTGACCATGAGGGGCTTGATTACATTGACCAGAAAATTTTGCG
TACCATGATTGAGGTTTATAACGGTGGTCCTGTCGGTTTAGGAACGCTATCCGTTAATATCGCTGAAGAGCGTGATACTG
TTGAGGATATGTATGAGCCGTATTTGATTCAAAAAGGCTTTATTATGCGCACTCGTACTGGTCGTGTGGCAACTGAAAAA
GCTTATGAACATTTGGGCTATCCTTATTCGGAAAAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (181-254)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (20-178)

• RuvB C-terminal winged helix domain (WHD_RuvB) (256-325)

3D structure

• AlphaFold DB: E0PGS8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Streptococcus pneumoniae TIGR4	89.157	100	0.892
ruvB	Streptococcus pneumoniae R6	89.157	100	0.892
ruvB	Streptococcus pneumoniae D39	89.157	100	0.892
ruvB	Bacillus subtilis subsp. subtilis str. 168	61.61	97.289	0.599
ruvB	Helicobacter pylori 26695	49.693	98.193	0.488
ruvB	Synechocystis sp. PCC 6803	49.521	94.277	0.467