Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGE   Type   Machinery gene
Locus tag   E8M05_RS00845 Genome accession   NZ_CP039457
Coordinates   121762..122055 (+) Length   97 a.a.
NCBI ID   WP_080543606.1    Uniprot ID   -
Organism   Streptococcus pasteurianus strain WUSP067     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 116762..127055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E8M05_RS00820 (E8M05_00815) - 118759..119121 (+) 363 WP_003062916.1 DUF1033 family protein -
  E8M05_RS00825 (E8M05_00820) comGA 119192..120133 (+) 942 WP_003062917.1 competence type IV pilus ATPase ComGA Machinery gene
  E8M05_RS00830 (E8M05_00825) comGB 120057..121100 (+) 1044 WP_003062919.1 competence type IV pilus assembly protein ComGB Machinery gene
  E8M05_RS00835 (E8M05_00830) comGC 121100..121393 (+) 294 WP_003062921.1 competence type IV pilus major pilin ComGC Machinery gene
  E8M05_RS00840 (E8M05_00835) comGD 121377..121808 (+) 432 WP_003062922.1 competence type IV pilus minor pilin ComGD Machinery gene
  E8M05_RS00845 (E8M05_00840) comGE 121762..122055 (+) 294 WP_080543606.1 competence type IV pilus minor pilin ComGE Machinery gene
  E8M05_RS00850 (E8M05_00845) comGF 122039..122476 (+) 438 WP_041974065.1 competence type IV pilus minor pilin ComGF Machinery gene
  E8M05_RS00855 (E8M05_00850) comGG 122448..122780 (+) 333 WP_003062925.1 competence type IV pilus minor pilin ComGG Machinery gene
  E8M05_RS00860 (E8M05_00855) comGH 122835..123806 (+) 972 WP_003062926.1 class I SAM-dependent methyltransferase Machinery gene
  E8M05_RS00865 (E8M05_00860) - 123844..125043 (+) 1200 WP_003062927.1 acetate kinase -
  E8M05_RS00870 (E8M05_00865) - 125204..125404 (+) 201 WP_136596396.1 helix-turn-helix transcriptional regulator -
  E8M05_RS00875 (E8M05_00870) - 125414..125623 (+) 210 WP_003062929.1 hypothetical protein -
  E8M05_RS00880 (E8M05_00875) - 125660..126088 (+) 429 WP_230321696.1 ABC transporter permease -
  E8M05_RS00885 (E8M05_00880) - 126100..126738 (+) 639 WP_003062931.1 CPBP family intramembrane glutamic endopeptidase -

Sequence

Protein


Download         Length: 97 a.a.        Molecular weight: 11069.10 Da        Isoelectric Point: 8.4757

>NTDB_id=317778 E8M05_RS00845 WP_080543606.1 121762..122055(+) (comGE) [Streptococcus pasteurianus strain WUSP067]
MVNIKRQKLKAYILLESLIALGLLAMITSIVLGEIDKNRQFMQESLRQQEVLNVATMAVQTGQNHLKMNGIEVEIIKKDG
EVYVYEAKTEILHVKKD

Nucleotide


Download         Length: 294 bp        

>NTDB_id=317778 E8M05_RS00845 WP_080543606.1 121762..122055(+) (comGE) [Streptococcus pasteurianus strain WUSP067]
GTGGTAAATATAAAAAGACAGAAACTAAAAGCTTACATACTCCTTGAAAGCTTGATAGCACTAGGATTGTTAGCGATGAT
TACGAGTATTGTTTTAGGGGAAATTGATAAAAATAGGCAGTTTATGCAAGAAAGTTTACGGCAGCAGGAAGTTCTTAATG
TGGCAACAATGGCTGTGCAAACAGGGCAAAATCATTTGAAGATGAATGGTATTGAGGTTGAGATTATCAAAAAAGATGGA
GAGGTCTATGTTTATGAGGCGAAAACGGAAATATTACATGTTAAAAAAGACTAG

Domains

Predicted by InterProScan.

Type II secretory pathway pseudopilin (ComGE)

(13-94)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGE Streptococcus mutans UA140

46.875

98.969

0.464
  comGE Streptococcus mutans UA159

46.875

98.969

0.464
  comGE/cglE Streptococcus mitis SK321

40.426

96.907

0.392
  comGE/cglE Streptococcus pneumoniae Rx1

40.426

96.907

0.392
  comGE/cglE Streptococcus pneumoniae D39

40.426

96.907

0.392
  comGE/cglE Streptococcus pneumoniae R6

40.426

96.907

0.392
  comGE/cglE Streptococcus pneumoniae TIGR4

40.426

96.907

0.392
  comGE/cglE Streptococcus mitis NCTC 12261

39.362

96.907

0.381

Multiple sequence alignment