Detailed information NTG0317038

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: D6Z43_RS03710
• Genome accession: NZ_CP032616
• Coordinates: 725542..726747 (+)
• Length: 401 a.a.
• NCBI ID: WP_174235570.1
• Uniprot ID: -
• Organism: Pseudomonas sp. DY-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 720542..731747

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D6Z43_RS03680 (D6Z43_03680)	-	721081..721587 (+)	507	WP_120650537.1	DUF1993 family protein	-
D6Z43_RS03685 (D6Z43_03685)	-	721786..722394 (+)	609	WP_120650539.1	FMN-dependent NADH-azoreductase	-
D6Z43_RS03690 (D6Z43_03690)	-	722587..724176 (-)	1590	WP_120650541.1	ABC-F family ATPase	-
D6Z43_RS03695 (D6Z43_03695)	-	724496..724681 (+)	186	WP_120650543.1	hypothetical protein	-
D6Z43_RS03700 (D6Z43_03700)	-	724768..725067 (+)	300	WP_120650545.1	hypothetical protein	-
D6Z43_RS03705 (D6Z43_03705)	-	725145..725423 (-)	279	WP_028627186.1	peptidylprolyl isomerase	-
D6Z43_RS03710 (D6Z43_03710)	pilU	725542..726747 (+)	1206	WP_174235570.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
D6Z43_RS03715 (D6Z43_03715)	-	727046..727642 (+)	597	WP_120650547.1	DJ-1/PfpI family protein	-
D6Z43_RS03720 (D6Z43_03720)	-	727766..729721 (-)	1956	WP_120650549.1	acetoacetate--CoA ligase	-
D6Z43_RS03725 (D6Z43_03725)	-	729781..730689 (-)	909	WP_120650550.1	LysR family transcriptional regulator	-
D6Z43_RS03730 (D6Z43_03730)	-	730815..731513 (+)	699	WP_120655174.1	CoA transferase subunit A	-

Sequence

Protein

• Length: 401 a.a.
• Molecular weight: 44282.61 Da
• Isoelectric Point: 6.1307

>NTDB_id=317038 D6Z43_RS03710 WP_174235570.1 725542..726747(+) (pilU) [Pseudomonas sp. DY-1]
MDLSAMLKILASQDGSDLYLSTGAPPCAKFNGLLRPLSNEPLKAGDVARIAESIMDPEQRAEFDRELEMNLAISLPNIGR
FRINIFRQRNEVSIVARNIKLDIPRFEDLKLPEVLLKMVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNTGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTSTISDLIKRGEFHSIKEIMEKSRGLGMQTFDQAL
FDLVVEGAIPEDEAIKNADSANNLRLKLKLYRDGPAAAPAPTAPAPTPVAAPSATQDAASWGLELKLEDLEEDGPEEPAP
R

Nucleotide

• Length: 1206 bp

>NTDB_id=317038 D6Z43_RS03710 WP_174235570.1 725542..726747(+) (pilU) [Pseudomonas sp. DY-1]
ATGGACCTCTCCGCGATGCTGAAAATCCTGGCCAGCCAGGATGGTTCCGACCTCTACCTGTCCACGGGCGCGCCGCCCTG
TGCCAAGTTCAACGGCCTGTTGCGGCCGCTCAGCAATGAGCCCCTGAAGGCCGGTGACGTGGCCAGGATCGCCGAATCGA
TCATGGACCCCGAGCAACGGGCCGAGTTCGACCGCGAACTGGAGATGAACCTGGCCATCTCGCTGCCGAACATCGGCCGC
TTCCGGATCAATATCTTCCGCCAGCGCAACGAGGTATCCATCGTCGCGCGGAACATCAAGCTGGATATCCCGCGCTTCGA
AGACCTCAAGCTGCCCGAAGTGCTGCTCAAGATGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGTGGCACTGGCT
CCGGTAAGTCCACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACACCGGCGGCCACATCATCACCATCGAAGAC
CCGGTGGAATACGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGGGTGGACACCCGCAGCTTCCACGC
CGCGCTGAAGAACACACTGCGCCAGGCGCCGGACGTGATTCTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCATG
CGCTGGCCTTCGCCGATACCGGTCACCTTGCGATCTCCACGCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAAGAACGTCGCCCGCAGTTGCTCAACGACCTGGGCAACAACCTCAAGGCTTTCGTTTCCCAGCG
CCTGGTGCGCACACAGGACGGCAAGCGCCGCGCGGCGGTGGAAGTGTTACTGGGTACCTCCACCATCAGCGACCTCATCA
AGCGCGGCGAATTCCACTCCATCAAGGAGATCATGGAGAAGTCCCGTGGCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTAGTGGAGGGCGCCATTCCCGAAGACGAAGCGATCAAGAACGCCGATTCGGCGAACAACCTGCGCCTGAA
GCTCAAGCTCTATCGCGATGGTCCTGCTGCAGCCCCAGCACCGACAGCCCCCGCCCCGACGCCAGTTGCTGCTCCGAGCG
CCACCCAGGATGCCGCCAGCTGGGGGCTGGAGCTGAAGCTGGAAGACCTGGAGGAGGATGGGCCGGAGGAGCCAGCGCCT
CGTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (112-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	87.531	0.494
pilU	Acinetobacter baylyi ADP1	53.409	87.781	0.469
pilU	Vibrio cholerae strain A1552	52.975	88.03	0.466
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.535	96.509	0.382