Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LLWM1_RS11995 Genome accession   NZ_CP032500
Coordinates   2334578..2335078 (-) Length   166 a.a.
NCBI ID   WP_003131953.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain WM1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2330642..2356401 2334578..2335078 within 0


Gene organization within MGE regions


Location: 2330642..2356401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLWM1_RS11970 (LLWM1_2282) gnd 2330642..2331547 (-) 906 WP_032398514.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  LLWM1_RS11975 (LLWM1_2283) - 2331911..2332759 (+) 849 WP_003131770.1 MurR/RpiR family transcriptional regulator -
  LLWM1_RS11980 (LLWM1_2284) - 2332797..2333105 (+) 309 WP_017865196.1 DUF960 domain-containing protein -
  LLWM1_RS11985 (LLWM1_2285) - 2333187..2333960 (-) 774 WP_003131951.1 metallophosphoesterase -
  LLWM1_RS11990 (LLWM1_2286) rpsR 2334160..2334405 (-) 246 WP_003131952.1 30S ribosomal protein S18 -
  LLWM1_RS11995 (LLWM1_2287) ssb 2334578..2335078 (-) 501 WP_003131953.1 single-stranded DNA-binding protein Machinery gene
  LLWM1_RS12000 (LLWM1_2288) rpsF 2335108..2335401 (-) 294 WP_003131954.1 30S ribosomal protein S6 -
  LLWM1_RS12005 (LLWM1_2289) - 2335593..2336447 (-) 855 WP_058221735.1 undecaprenyl-diphosphate phosphatase -
  LLWM1_RS12010 (LLWM1_2290) - 2336970..2338436 (+) 1467 WP_017865200.1 amino acid permease -
  LLWM1_RS12015 (LLWM1_2291) dnaX 2338471..2340129 (-) 1659 WP_058221736.1 DNA polymerase III subunit gamma/tau -
  LLWM1_RS12020 (LLWM1_2292) - 2340178..2340819 (-) 642 WP_003131958.1 phosphotransferase -
  LLWM1_RS12025 (LLWM1_2293) - 2340850..2341338 (-) 489 WP_003131959.1 GAF domain-containing protein -
  LLWM1_RS12030 (LLWM1_2294) - 2341422..2341694 (+) 273 WP_003131960.1 chorismate mutase -
  LLWM1_RS12035 (LLWM1_2295) - 2341782..2342228 (-) 447 WP_003131961.1 hypothetical protein -
  LLWM1_RS12040 (LLWM1_2296) - 2342518..2343702 (-) 1185 WP_058221737.1 site-specific integrase -
  LLWM1_RS12045 (LLWM1_2297) - 2344201..2344395 (-) 195 WP_058221577.1 hypothetical protein -
  LLWM1_RS12050 (LLWM1_2298) - 2345199..2345513 (-) 315 WP_011835952.1 hypothetical protein -
  LLWM1_RS12055 (LLWM1_2299) - 2346248..2347192 (-) 945 WP_058221334.1 Abi family protein -
  LLWM1_RS12060 (LLWM1_2300) - 2347653..2347847 (-) 195 WP_021212116.1 hypothetical protein -
  LLWM1_RS12065 (LLWM1_03275) - 2348483..2348674 (-) 192 Protein_2342 helix-turn-helix domain-containing protein -
  LLWM1_RS12070 (LLWM1_2302) - 2348934..2349131 (+) 198 WP_011677058.1 hypothetical protein -
  LLWM1_RS12075 (LLWM1_03280) - 2349135..2349383 (+) 249 WP_058221815.1 hypothetical protein -
  LLWM1_RS12080 (LLWM1_2303) - 2349498..2349692 (+) 195 WP_058221814.1 DUF1655 domain-containing protein -
  LLWM1_RS12085 (LLWM1_2304) - 2349696..2350220 (+) 525 WP_082225264.1 hypothetical protein -
  LLWM1_RS12090 (LLWM1_2305) - 2350217..2350468 (+) 252 WP_082225265.1 helix-turn-helix transcriptional regulator -
  LLWM1_RS12095 (LLWM1_2306) - 2350465..2350746 (+) 282 WP_082225266.1 hypothetical protein -
  LLWM1_RS12100 (LLWM1_2307) - 2350789..2351028 (+) 240 WP_058221371.1 hypothetical protein -
  LLWM1_RS12105 (LLWM1_2308) - 2351025..2351279 (+) 255 WP_039114370.1 hypothetical protein -
  LLWM1_RS12110 (LLWM1_2309) - 2351276..2351470 (+) 195 WP_047687087.1 hypothetical protein -
  LLWM1_RS12115 (LLWM1_2310) - 2351457..2351786 (+) 330 WP_058221372.1 hypothetical protein -
  LLWM1_RS12120 (LLWM1_2311) - 2351790..2352584 (+) 795 WP_082225267.1 bifunctional DNA primase/polymerase -
  LLWM1_RS12125 (LLWM1_2312) - 2352595..2354229 (+) 1635 WP_082225268.1 phage/plasmid primase, P4 family -
  LLWM1_RS12130 (LLWM1_2313) - 2354442..2354624 (+) 183 WP_003131978.1 hypothetical protein -
  LLWM1_RS12135 (LLWM1_2314) - 2354651..2355259 (+) 609 WP_003131979.1 hypothetical protein -
  LLWM1_RS12140 (LLWM1_2315) - 2355268..2355858 (+) 591 WP_003131980.1 HK97 family phage prohead protease -
  LLWM1_RS12145 (LLWM1_2316) - 2356072..2356401 (+) 330 WP_003131981.1 hypothetical protein -

Sequence


Protein


Download         Length: 166 a.a.        Molecular weight: 18304.13 Da        Isoelectric Point: 5.2279

>NTDB_id=316490 LLWM1_RS11995 WP_003131953.1 2334578..2335078(-) (ssb) [Lactococcus lactis subsp. lactis strain WM1]
MINNVVLVGRITRDPELRYTPQNQAVATFSLAVNRQFKNANGEREADFINCVIWRQQAENLANWAKKGALIGVTGRIQTR
NYENQQGQRVYVTEVVADSFQMLESRSAREGMGGGTSAGSYSAPSQSTNNTPRPQTNNNNATPNFGRDADPFGSSPMEIS
DDDLPF

Nucleotide


Download         Length: 501 bp        

>NTDB_id=316490 LLWM1_RS11995 WP_003131953.1 2334578..2335078(-) (ssb) [Lactococcus lactis subsp. lactis strain WM1]
ATGATTAACAATGTTGTATTAGTAGGACGCATTACTCGCGATCCTGAACTTCGATACACCCCTCAAAATCAAGCTGTCGC
AACATTTTCATTGGCTGTAAACCGTCAATTTAAAAATGCGAATGGCGAACGTGAGGCTGATTTCATTAACTGCGTTATTT
GGCGCCAACAAGCTGAGAATTTGGCGAATTGGGCTAAAAAAGGAGCTTTGATTGGGGTAACTGGTCGAATTCAAACACGT
AACTATGAAAATCAACAAGGTCAACGCGTTTATGTGACTGAGGTTGTGGCTGACAGTTTCCAAATGTTGGAAAGTAGATC
TGCTCGTGAAGGTATGGGAGGCGGAACTTCTGCTGGTTCATATTCTGCACCTAGCCAATCTACAAATAATACTCCACGTC
CACAAACGAATAACAATAATGCAACACCGAATTTCGGTCGTGATGCTGATCCGTTTGGTAGCTCACCGATGGAAATTTCG
GATGATGACCTTCCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60.465

100

0.627

  ssbA Bacillus subtilis subsp. subtilis str. 168

57.803

100

0.602

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

63.855

0.373

  ssb Glaesserella parasuis strain SC1401

35.028

100

0.373


Multiple sequence alignment