Detailed information NTG0315893

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: LLB26_RS12765
• Genome accession: NZ_CP032435
• Coordinates: 1071429..1072214 (-)
• Length: 261 a.a.
• NCBI ID: WP_228763967.1
• Uniprot ID: A0AAJ6MIS5
• Organism: Lactococcus cremoris strain B26
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1066429..1077214

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLB26_RS05280 (LLB26_1040)	-	1067082..1068035 (+)	954	WP_015082369.1	PhoH family protein	-
LLB26_RS05285 (LLB26_1041)	-	1068105..1068599 (+)	495	WP_015082370.1	NUDIX hydrolase	-
LLB26_RS05290 (LLB26_1042)	ybeY	1068690..1069178 (+)	489	WP_011835335.1	rRNA maturation RNase YbeY	-
LLB26_RS05295 (LLB26_1043)	-	1069162..1069617 (+)	456	WP_015082371.1	diacylglycerol kinase	-
LLB26_RS05300 (LLB26_1044)	-	1069634..1070212 (+)	579	WP_015082372.1	nucleotidyltransferase family protein	-
LLB26_RS05305 (LLB26_1045)	comFC	1070270..1070920 (-)	651	WP_015082373.1	ComF family protein	Machinery gene
LLB26_RS12760 (LLB26_1046)	comFA	1070920..1071432 (-)	513	WP_228763816.1	helicase-related protein	Machinery gene
LLB26_RS12765 (LLB26_1047)	comFA	1071429..1072214 (-)	786	WP_228763967.1	DEAD/DEAH box helicase family protein	Machinery gene
LLB26_RS05315 (LLB26_1048)	-	1072271..1072906 (+)	636	WP_015082374.1	YigZ family protein	-
LLB26_RS05320 (LLB26_1049)	-	1072948..1073448 (+)	501	WP_015082375.1	VanZ family protein	-
LLB26_RS05325 (LLB26_1050)	-	1073535..1074533 (+)	999	WP_237025417.1	DUF475 domain-containing protein	-
LLB26_RS05330 (LLB26_1051)	-	1074642..1075409 (+)	768	WP_011676099.1	5'-nucleotidase	-
LLB26_RS05335 (LLB26_1052)	-	1075646..1076293 (+)	648	WP_011676100.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 261 a.a.
• Molecular weight: 29903.55 Da
• Isoelectric Point: 7.8602

>NTDB_id=315893 LLB26_RS12765 WP_228763967.1 1071429..1072214(-) (comFA) [Lactococcus cremoris strain B26]
MNLILENLLGRLLLEKDISAFYNFTDQVNNENKLIKICAMTSVNANKVRCNRCGTIHIKTNVKLPIGAFFCPTCLELGRV
RSDEYFYHLPQQDFSEKTYLRWTGKLTENQEKISNALCQQITNNQKRLVQAVTGAGKTEMIYQLIEQILSHGGSVGLASP
RIDVCIELHQRLSRDFTCQIPLLYHEGDSYFRSPLVVMTSHQLLRFKEAFDLLIIDEVDAFPFRDNDMLFFALENAKKIN
GNLLYLTATSTDKLDKQIKKA

Nucleotide

• Length: 786 bp

>NTDB_id=315893 LLB26_RS12765 WP_228763967.1 1071429..1072214(-) (comFA) [Lactococcus cremoris strain B26]
ATGAACTTAATTTTAGAAAATCTCCTTGGGCGTCTTTTACTAGAAAAAGATATTTCAGCTTTTTATAATTTTACTGACCA
AGTTAACAATGAAAACAAATTAATTAAAATTTGTGCAATGACATCTGTCAATGCTAATAAAGTAAGATGTAATCGATGTG
GCACGATTCATATAAAAACCAATGTTAAACTTCCGATTGGTGCTTTTTTCTGTCCAACATGTCTTGAACTTGGTCGTGTC
CGCTCAGATGAATATTTCTATCATCTCCCTCAACAAGACTTTTCAGAAAAAACTTACTTACGTTGGACAGGAAAATTAAC
AGAAAATCAAGAAAAAATTTCTAACGCTCTCTGTCAGCAAATTACTAATAATCAAAAACGACTTGTTCAAGCCGTTACTG
GTGCTGGTAAAACAGAAATGATATATCAGCTTATTGAACAAATTCTTAGCCATGGGGGTTCGGTCGGTTTAGCCAGCCCA
AGAATTGATGTATGCATTGAGCTTCATCAACGTTTATCACGTGATTTCACTTGTCAAATTCCCCTTTTGTATCATGAGGG
AGATTCTTATTTTCGTTCGCCTTTGGTCGTTATGACCAGTCACCAACTTTTACGTTTCAAAGAGGCCTTTGATTTGCTTA
TCATCGATGAAGTCGATGCCTTTCCTTTTAGAGATAATGACATGCTTTTTTTCGCTCTTGAAAATGCTAAAAAAATTAAT
GGAAACTTGCTCTACTTAACCGCTACATCTACTGACAAATTAGACAAACAGATAAAAAAAGCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (105-249)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	96.923	99.617	0.966
comFA/cflA	Streptococcus mitis SK321	49.407	96.935	0.479
comFA/cflA	Streptococcus pneumoniae D39	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae Rx1	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae R6	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae TIGR4	48.617	96.935	0.471
comFA/cflA	Streptococcus mitis NCTC 12261	48.221	96.935	0.467
comFA	Bacillus subtilis subsp. subtilis str. 168	37.453	100	0.383