Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   E5M00_RS21005 Genome accession   NZ_CP038791
Coordinates   4197105..4197545 (+) Length   146 a.a.
NCBI ID   WP_000360904.1    Uniprot ID   P36647
Organism   Escherichia coli strain PF9285     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4192105..4202545
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5M00_RS20985 (E5M00_20985) aroP 4193103..4194476 (+) 1374 WP_000969915.1 aromatic amino acid transporter AroP -
  E5M00_RS20990 (E5M00_20990) ampE 4194519..4195373 (-) 855 WP_000172005.1 beta-lactamase regulator AmpE -
  E5M00_RS20995 (E5M00_20995) ampD 4195370..4195921 (-) 552 WP_000923721.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  E5M00_RS21000 (E5M00_21000) nadC 4196009..4196902 (+) 894 WP_001135174.1 carboxylating nicotinate-nucleotide diphosphorylase -
  E5M00_RS21005 (E5M00_21005) pilA 4197105..4197545 (+) 441 WP_000360904.1 prepilin peptidase-dependent pilin Machinery gene
  E5M00_RS21010 (E5M00_21010) pilB 4197555..4198940 (+) 1386 WP_001025145.1 type II secretion system protein GspE Machinery gene
  E5M00_RS21015 (E5M00_21015) hofC 4198930..4200132 (+) 1203 WP_000157266.1 protein transport protein HofC -
  E5M00_RS21020 (E5M00_21020) guaC 4200167..4201210 (-) 1044 WP_001217338.1 GMP reductase -
  E5M00_RS27600 - 4201366..4201410 (-) 45 WP_120795372.1 protein YacM -
  E5M00_RS21025 (E5M00_21025) coaE 4201435..4202055 (+) 621 WP_001269520.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15621.75 Da        Isoelectric Point: 4.3938

>NTDB_id=315809 E5M00_RS21005 WP_000360904.1 4197105..4197545(+) (pilA) [Escherichia coli strain PF9285]
MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQTFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTR
YVSAMSVAKGVVSLTGQESLNGLSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN

Nucleotide


Download         Length: 441 bp        

>NTDB_id=315809 E5M00_RS21005 WP_000360904.1 4197105..4197545(+) (pilA) [Escherichia coli strain PF9285]
ATGGACAAGCAACGCGGTTTTACACTTATCGAACTGATGGTGGTTATTGGCATCATTGCCATTTTAAGCGCCATTGGTAT
TCCCGCTTATCAAAACTACCTGCGCAAAGCCGCACTCACCGACATGCTACAAACCTTTGTGCCTTACCGTACCGCCGTAG
AGTTGTGCGCGCTGGAACATGGTGGATTAGATACCTGCGACGGTGGCAGCAATGGCATTCCCTCGCCTACCACCACCCGC
TATGTTTCAGCCATGAGTGTGGCAAAGGGCGTGGTGTCGCTGACCGGGCAAGAAAGTCTCAATGGGCTAAGCGTCGTCAT
GACACCGGGTTGGGATAACGCAAACGGCGTCACCGGCTGGACGCGCAACTGCAATATTCAAAGTGACAGCGCATTGCAGC
AAGCCTGCGAAGATGTCTTCCGCTTTGATGACGCCAACTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-25)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P36647

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

43.089

84.247

0.363

Multiple sequence alignment