Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   D2M30_RS12575 Genome accession   NZ_CP032146
Coordinates   2499626..2500696 (-) Length   356 a.a.
NCBI ID   WP_125122960.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain YP6     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2494626..2505696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D2M30_RS12525 (D2M30_12550) sipW 2494685..2495269 (-) 585 WP_125122955.1 signal peptidase I SipW -
  D2M30_RS12530 (D2M30_12555) tapA 2495241..2495912 (-) 672 WP_125122956.1 amyloid fiber anchoring/assembly protein TapA -
  D2M30_RS12535 (D2M30_12560) - 2496170..2496499 (+) 330 WP_013352865.1 DUF3889 domain-containing protein -
  D2M30_RS12540 (D2M30_12565) - 2496540..2496719 (-) 180 WP_016938971.1 YqzE family protein -
  D2M30_RS12545 (D2M30_12570) comGG 2496776..2497153 (-) 378 WP_071348016.1 competence type IV pilus minor pilin ComGG Machinery gene
  D2M30_RS12550 (D2M30_12575) comGF 2497155..2497559 (-) 405 WP_185715963.1 competence type IV pilus minor pilin ComGF -
  D2M30_RS12555 (D2M30_12580) comGE 2497564..2497878 (-) 315 WP_125122957.1 competence type IV pilus minor pilin ComGE Machinery gene
  D2M30_RS12560 (D2M30_12585) comGD 2497862..2498299 (-) 438 WP_125122958.1 competence type IV pilus minor pilin ComGD Machinery gene
  D2M30_RS12565 (D2M30_12590) comGC 2498289..2498597 (-) 309 WP_013352870.1 competence type IV pilus major pilin ComGC Machinery gene
  D2M30_RS12570 (D2M30_12595) comGB 2498602..2499639 (-) 1038 WP_125122959.1 competence type IV pilus assembly protein ComGB Machinery gene
  D2M30_RS12575 (D2M30_12600) comGA 2499626..2500696 (-) 1071 WP_125122960.1 competence type IV pilus ATPase ComGA Machinery gene
  D2M30_RS12580 (D2M30_12605) - 2500890..2501839 (-) 950 Protein_2448 magnesium transporter CorA family protein -
  D2M30_RS12585 (D2M30_12610) - 2501986..2503287 (+) 1302 WP_088613120.1 hemolysin family protein -
  D2M30_RS12590 (D2M30_12615) - 2503325..2504155 (-) 831 WP_071348011.1 STAS domain-containing protein -
  D2M30_RS12600 (D2M30_12625) - 2504368..2504748 (-) 381 WP_013352876.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  D2M30_RS12605 (D2M30_12630) - 2504984..2505229 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40512.86 Da        Isoelectric Point: 9.4729

>NTDB_id=314032 D2M30_RS12575 WP_125122960.1 2499626..2500696(-) (comGA) [Bacillus amyloliquefaciens strain YP6]
MENIETYSRNIINEAYMARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPKETVHLRMSTLPTMNDESLVIRLLPKRQIPPLDKLSLFPGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSSGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKTDNCSVYCRLYRKTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=314032 D2M30_RS12575 WP_125122960.1 2499626..2500696(-) (comGA) [Bacillus amyloliquefaciens strain YP6]
ATGGAGAATATTGAAACATACAGCAGGAACATCATCAACGAGGCCTATATGGCAAGGGCTTCAGATATACACATTGTTCC
GAGGGAAAGGGATGCTTTCATTCATTTTCGCATCGGTCATGCCCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTTAAATTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAGAACGGATCACTGTCT
CTGCCGCTTCCAAAGGAAACCGTTCATTTGAGGATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
ATTGCCTAAGAGGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCCGGGGGCAGGCGCCGCATTATTATCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTTTTTACCGGCCCGACCGGATCTGGAAAAACAACCACCCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTGGAAGATCCCGTAGAAACGAGAGATGAAGATGTTTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCTCCGGGCTAAAAGCGATTTTAAGACATGACCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTATTGTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTCGGGGTGAACATGACGGAAATTGAACAAACCGTTATTGC
CATCGCCGCTCAGCGTCTGGTTGATTTAACGTGTCCGTTTTGCAAAACGGACAACTGTTCCGTGTACTGCCGTCTGTACA
GGAAGACACGCCGTGCGGGGATTTATGAACTGCTTTACGGAAAAAATCTCACCCAGTGCTTTCAAGAAGCAAAAGGAGAG
CACGCCAATTTTCAATATCAGACGCTTCGCAGGCTGATCAGAAAAGGTATCGCGCTCGGTTATGTCACGACTGACAATTA
TGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.506

97.753

0.376

Multiple sequence alignment