Detailed information NTG0312407

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: D1E88_RS07380
• Genome accession: NZ_CP031886
• Coordinates: 1531301..1531798 (-)
• Length: 165 a.a.
• NCBI ID: WP_001796472.1
• Uniprot ID: A0A5F0HJ55
• Organism: Staphylococcus aureus strain CFSAN082783
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1526301..1536798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
D1E88_RS07355 (D1E88_07520)	gcvPB	1526463..1527935 (-)	1473	WP_000202176.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
D1E88_RS07360 (D1E88_07525)	gcvPA	1527928..1529274 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
D1E88_RS07365 (D1E88_07530)	gcvT	1529294..1530385 (-)	1092	WP_000093344.1	glycine cleavage system aminomethyltransferase GcvT	-
D1E88_RS07370 (D1E88_07535)	-	1530544..1531068 (-)	525	WP_001015120.1	shikimate kinase	-
D1E88_RS07375 (D1E88_07540)	-	1531058..1531204 (-)	147	WP_001789879.1	hypothetical protein	-
D1E88_RS07380 (D1E88_07545)	comGF	1531301..1531798 (-)	498	WP_001796472.1	competence type IV pilus minor pilin ComGF	Machinery gene
D1E88_RS07385 (D1E88_07550)	comGE	1531716..1532015 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
D1E88_RS07390 (D1E88_07555)	comGD	1532002..1532448 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
D1E88_RS07395 (D1E88_07560)	comGC	1532426..1532737 (-)	312	WP_000472255.1	competence type IV pilus major pilin ComGC	Machinery gene
D1E88_RS07400 (D1E88_07565)	comGB	1532751..1533821 (-)	1071	WP_000776409.1	competence type IV pilus assembly protein ComGB	Machinery gene
D1E88_RS07405 (D1E88_07570)	comGA	1533793..1534767 (-)	975	WP_000697223.1	competence type IV pilus ATPase ComGA	Machinery gene
D1E88_RS07410 (D1E88_07575)	-	1534819..1535442 (-)	624	WP_001223009.1	MBL fold metallo-hydrolase	-
D1E88_RS07415 (D1E88_07580)	-	1535439..1535768 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
D1E88_RS07420 (D1E88_07585)	-	1535768..1536754 (-)	987	WP_000161307.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19169.89 Da
• Isoelectric Point: 10.1358

>NTDB_id=312407 D1E88_RS07380 WP_001796472.1 1531301..1531798(-) (comGF) [Staphylococcus aureus strain CFSAN082783]
MILSKVTNKYVPFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=312407 D1E88_RS07380 WP_001796472.1 1531301..1531798(-) (comGF) [Staphylococcus aureus strain CFSAN082783]
ATGATATTAAGCAAAGTGACCAACAAATATGTGCCATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (68-155)

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

3D structure

• AlphaFold DB: A0A5F0HJ55

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	95.758	100	0.958