Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   D1095_RS04325 Genome accession   NZ_CP031875
Coordinates   973728..974930 (-) Length   400 a.a.
NCBI ID   WP_005601207.1    Uniprot ID   Q7BI81
Organism   Actinobacillus pleuropneumoniae serovar 2 str. S1536     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 968728..979930
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1095_RS04300 (D1095_04365) lptE 970528..971022 (+) 495 WP_005597385.1 LPS assembly lipoprotein LptE -
  D1095_RS04305 (D1095_04370) holA 971131..972156 (+) 1026 WP_005601200.1 DNA polymerase III subunit delta -
  D1095_RS04310 (D1095_04375) yacG 972233..972421 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1095_RS04315 (D1095_04380) coaE 972403..973041 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1095_RS04320 (D1095_04385) - 973063..973701 (-) 639 WP_005604550.1 prepilin peptidase -
  D1095_RS04325 (D1095_04390) pilC 973728..974930 (-) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  D1095_RS04330 (D1095_04395) pilB 974914..976308 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1095_RS04335 (D1095_04400) pilA 976326..976772 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1095_RS04340 (D1095_04405) radA 976954..978333 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1095_RS04345 (D1095_04410) - 978377..978610 (-) 234 WP_005597399.1 YgjV family protein -
  D1095_RS04350 (D1095_04415) folA 978737..979225 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 45588.09 Da        Isoelectric Point: 10.3239

>NTDB_id=312284 D1095_RS04325 WP_005601207.1 973728..974930(-) (pilC) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MLKVYEFHWKAKNRFQQLQKGKALACHRTELERRLLAKGYSQIRIQRNFRFVTQASSESITQVIKQLALLLNAAVPLKQA
LNMLLENTYHIRIYMWLNDLNRLIESGYTMSSSLTKLNLYLAKSEIQLVRIGEQSGRLGEILQNIAVNRHQTEKLHKKIK
KIMFYPVVTLIISVALSLAMLLFIVPQFIELYSGREKPLPLITQMLFTLSHFLQHSLLQVTIVSLLAVGFFRFFAKKYRF
LTAIKNGMLSALPIFNKIVENVRIVFFSQNLGLMLKAHIRLETALNTFLAAQSQDLRLQKEIENILAQLKQGYRFSESIN
ASVFGTQVVQMLAIGEQSGNVAQMCSYIGELYRQQLDDQIELLSQLLEPILMLIIGLIIGTVLIGLYLPIFDLGGLVGGG

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=312284 D1095_RS04325 WP_005601207.1 973728..974930(-) (pilC) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGTTGAAAGTGTATGAATTTCATTGGAAAGCTAAAAATCGTTTTCAACAACTTCAGAAAGGAAAAGCGTTAGCTTGTCA
TCGAACGGAATTGGAACGGCGTTTATTAGCAAAAGGCTATTCACAGATCCGTATCCAACGTAATTTTCGTTTTGTCACAC
AGGCTTCTTCAGAATCGATTACTCAAGTAATTAAACAGTTGGCGCTTTTACTGAATGCCGCAGTCCCTTTAAAACAAGCG
CTGAATATGTTGTTAGAAAATACTTATCATATTCGGATCTATATGTGGCTGAATGATTTAAACCGTTTGATAGAATCGGG
TTATACAATGTCGTCAAGTTTAACCAAATTGAATTTATATTTGGCGAAATCGGAAATACAGCTGGTACGGATTGGCGAAC
AAAGCGGGCGATTAGGCGAAATTTTGCAAAATATTGCGGTAAATCGCCATCAAACCGAAAAATTACATAAGAAAATAAAA
AAGATAATGTTTTATCCTGTCGTCACTTTGATTATTTCCGTTGCGCTTTCTCTCGCCATGCTCCTTTTTATCGTGCCGCA
ATTTATCGAGCTGTATTCCGGTCGTGAAAAGCCGCTTCCTTTAATTACACAAATGCTATTTACATTATCGCACTTCTTAC
AACATTCTTTATTACAGGTAACGATAGTCAGTTTATTAGCGGTCGGTTTTTTCCGTTTTTTTGCAAAAAAATATCGATTT
TTAACCGCTATAAAAAACGGGATGTTAAGTGCTTTGCCTATTTTTAATAAGATAGTCGAAAACGTTCGTATAGTATTTTT
TAGTCAAAATCTCGGCTTGATGCTAAAAGCTCATATTCGGCTGGAAACGGCGTTAAATACCTTTCTTGCTGCACAATCGC
AAGATTTGAGGTTACAAAAAGAGATAGAAAATATTTTAGCGCAATTAAAACAAGGTTATCGCTTTTCCGAAAGTATTAAT
GCGAGTGTGTTCGGTACTCAAGTAGTACAAATGCTTGCCATTGGCGAACAGAGTGGGAATGTAGCGCAGATGTGTAGCTA
TATCGGGGAGTTATATCGACAACAATTGGATGATCAGATTGAACTGTTATCGCAGCTGCTTGAGCCGATCTTAATGCTGA
TTATCGGATTAATTATCGGTACGGTTTTGATCGGATTATATTTACCGATTTTTGATTTAGGCGGACTAGTCGGAGGCGGT
TAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(267-389)

Type II secretion system (T2SS), protein F (T2SSF)

(64-186)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7BI81

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Glaesserella parasuis strain SC1401

50.126

99.25

0.498
  pilC Haemophilus influenzae Rd KW20

39.5

100

0.395
  pilC Haemophilus influenzae 86-028NP

38.5

100

0.385

Multiple sequence alignment