Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   D1095_RS04335 Genome accession   NZ_CP031875
Coordinates   976326..976772 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae serovar 2 str. S1536     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 971326..981772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1095_RS04310 (D1095_04375) yacG 972233..972421 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1095_RS04315 (D1095_04380) coaE 972403..973041 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1095_RS04320 (D1095_04385) - 973063..973701 (-) 639 WP_005604550.1 prepilin peptidase -
  D1095_RS04325 (D1095_04390) pilC 973728..974930 (-) 1203 WP_005601207.1 type II secretion system F family protein Machinery gene
  D1095_RS04330 (D1095_04395) pilB 974914..976308 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1095_RS04335 (D1095_04400) pilA 976326..976772 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1095_RS04340 (D1095_04405) radA 976954..978333 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1095_RS04345 (D1095_04410) - 978377..978610 (-) 234 WP_005597399.1 YgjV family protein -
  D1095_RS04350 (D1095_04415) folA 978737..979225 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1095_RS04355 (D1095_04420) proB 979312..980403 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1095_RS04360 (D1095_04425) - 980416..981192 (+) 777 WP_005601215.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=312286 D1095_RS04335 WP_005597397.1 976326..976772(-) (pilA) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=312286 D1095_RS04335 WP_005597397.1 976326..976772(-) (pilA) [Actinobacillus pleuropneumoniae serovar 2 str. S1536]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment