Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1096_RS04340 Genome accession   NZ_CP031874
Coordinates   978645..979091 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain S1421     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973645..984091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1096_RS04315 (D1096_04330) yacG 974552..974740 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1096_RS04320 (D1096_04335) coaE 974722..975360 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1096_RS04325 (D1096_04340) - 975382..976020 (-) 639 WP_005604550.1 prepilin peptidase -
  D1096_RS04330 (D1096_04345) pilC 976047..977249 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  D1096_RS04335 (D1096_04350) pilB 977233..978627 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1096_RS04340 (D1096_04355) pilA 978645..979091 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1096_RS04345 (D1096_04360) radA 979273..980652 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1096_RS04350 (D1096_04365) - 980696..980929 (-) 234 WP_005597399.1 YgjV family protein -
  D1096_RS04355 (D1096_04370) folA 981056..981544 (-) 489 WP_012263007.1 type 3 dihydrofolate reductase -
  D1096_RS04360 (D1096_04375) proB 981631..982722 (+) 1092 WP_237586572.1 glutamate 5-kinase -
  D1096_RS04365 (D1096_04380) - 982735..983511 (+) 777 WP_237586573.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=312259 D1096_RS04340 WP_005597397.1 978645..979091(-) (pilA) [Actinobacillus pleuropneumoniae strain S1421]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=312259 D1096_RS04340 WP_005597397.1 978645..979091(-) (pilA) [Actinobacillus pleuropneumoniae strain S1421]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547
  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527

Multiple sequence alignment