Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   D1096_RS04345 Genome accession   NZ_CP031874
Coordinates   979273..980652 (+) Length   459 a.a.
NCBI ID   WP_005601211.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain S1421     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 974273..985652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1096_RS04315 (D1096_04330) yacG 974552..974740 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1096_RS04320 (D1096_04335) coaE 974722..975360 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1096_RS04325 (D1096_04340) - 975382..976020 (-) 639 WP_005604550.1 prepilin peptidase -
  D1096_RS04330 (D1096_04345) pilC 976047..977249 (-) 1203 WP_012263006.1 type II secretion system F family protein Machinery gene
  D1096_RS04335 (D1096_04350) pilB 977233..978627 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1096_RS04340 (D1096_04355) pilA 978645..979091 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1096_RS04345 (D1096_04360) radA 979273..980652 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1096_RS04350 (D1096_04365) - 980696..980929 (-) 234 WP_005597399.1 YgjV family protein -
  D1096_RS04355 (D1096_04370) folA 981056..981544 (-) 489 WP_012263007.1 type 3 dihydrofolate reductase -
  D1096_RS04360 (D1096_04375) proB 981631..982722 (+) 1092 WP_237586572.1 glutamate 5-kinase -
  D1096_RS04365 (D1096_04380) - 982735..983511 (+) 777 WP_237586573.1 TSUP family transporter -
  D1096_RS04370 (D1096_04385) nth 983512..984144 (+) 633 WP_005597404.1 endonuclease III -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49678.24 Da        Isoelectric Point: 7.9094

>NTDB_id=312260 D1096_RS04345 WP_005601211.1 979273..980652(+) (radA) [Actinobacillus pleuropneumoniae strain S1421]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=312260 D1096_RS04345 WP_005601211.1 979273..980652(+) (radA) [Actinobacillus pleuropneumoniae strain S1421]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATTAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGTGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACTGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTATTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCCGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427

Multiple sequence alignment