Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   D1105_RS04470 Genome accession   NZ_CP031862
Coordinates   997765..998211 (-) Length   148 a.a.
NCBI ID   WP_005615188.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain 8329     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 992765..1003211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1105_RS04445 (D1105_04450) yacG 993672..993860 (-) 189 WP_005597388.1 DNA gyrase inhibitor YacG -
  D1105_RS04450 (D1105_04455) coaE 993842..994480 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1105_RS04455 (D1105_04460) - 994502..995140 (-) 639 WP_005604550.1 prepilin peptidase -
  D1105_RS04460 (D1105_04465) pilC 995167..996369 (-) 1203 WP_005612209.1 type II secretion system F family protein Machinery gene
  D1105_RS04465 (D1105_04470) pilB 996353..997747 (-) 1395 WP_005615186.1 GspE/PulE family protein Machinery gene
  D1105_RS04470 (D1105_04475) pilA 997765..998211 (-) 447 WP_005615188.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1105_RS04475 (D1105_04480) radA 998393..999772 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1105_RS04480 (D1105_04485) - 999816..1000049 (-) 234 WP_005615190.1 YgjV family protein -
  D1105_RS04485 (D1105_04490) folA 1000176..1000664 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1105_RS04490 (D1105_04495) proB 1000751..1001842 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1105_RS04495 (D1105_04500) - 1001855..1002631 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15953.36 Da        Isoelectric Point: 7.6257

>NTDB_id=312076 D1105_RS04470 WP_005615188.1 997765..998211(-) (pilA) [Actinobacillus pleuropneumoniae strain 8329]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHSGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=312076 D1105_RS04470 WP_005615188.1 997765..998211(-) (pilA) [Actinobacillus pleuropneumoniae strain 8329]
ATGCAAAAATTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAGCGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689
  pilA Haemophilus influenzae 86-028NP

54.422

99.324

0.541
  pilA Haemophilus influenzae Rd KW20

52.381

99.324

0.52

Multiple sequence alignment