Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   D1105_RS01045 Genome accession   NZ_CP031862
Coordinates   223857..225155 (+) Length   432 a.a.
NCBI ID   WP_005614439.1    Uniprot ID   A0A3G2M2X7
Organism   Actinobacillus pleuropneumoniae strain 8329     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218857..230155
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1105_RS01020 (D1105_01020) - 219029..221596 (-) 2568 WP_005614417.1 penicillin-binding protein 1A -
  D1105_RS01025 (D1105_01025) - 221735..222415 (+) 681 WP_005614420.1 hypothetical protein -
  D1105_RS01030 (D1105_01030) - 222397..222918 (+) 522 WP_043995260.1 hypothetical protein -
  D1105_RS01035 (D1105_01035) - 223017..223448 (+) 432 WP_043995262.1 hypothetical protein -
  D1105_RS01040 (D1105_01040) - 223441..223845 (+) 405 WP_005614437.1 hypothetical protein -
  D1105_RS01045 (D1105_01045) comE 223857..225155 (+) 1299 WP_005614439.1 type IV pilus secretin PilQ Machinery gene
  D1105_RS01050 (D1105_01050) nusB 225281..225694 (+) 414 WP_005595979.1 transcription antitermination factor NusB -
  D1105_RS01055 (D1105_01055) thiL 225759..226724 (+) 966 WP_005614441.1 thiamine-phosphate kinase -
  D1105_RS01060 (D1105_01060) - 226737..227210 (+) 474 WP_005595983.1 phosphatidylglycerophosphatase A -
  D1105_RS01065 (D1105_01065) - 227219..227845 (+) 627 WP_237611553.1 LysE family transporter -
  D1105_RS01070 (D1105_01070) - 227907..228947 (-) 1041 WP_005614445.1 TrmH family RNA methyltransferase -
  D1105_RS01075 (D1105_01075) suhB 229113..229925 (+) 813 WP_005595989.1 inositol-1-monophosphatase -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 47775.96 Da        Isoelectric Point: 9.5568

>NTDB_id=312069 D1105_RS01045 WP_005614439.1 223857..225155(+) (comE) [Actinobacillus pleuropneumoniae strain 8329]
MRKMIWLLLFILTPVMANTFSLSLKNAPTAEILSYLAEEYGKNIVLSDNIETNTTLRIENSDFDSVLKSITRANKLTSAY
EKQIYFIGHKKDEKAATAIGVNSDLLKPKLITKTIKLDYAKAAEVIESLTKGSGHFLSENGYLHFDDRSNSLIIKDSPES
MKNIVQLIRNLDKPTEQIAIEARIVTISSENLQELGVRWGIFAPTNGHHKIAGSLEANGLPNTNRLNVNFPVNNAASIAL
QVAKINGRVLDLELTALEQENNVEIIASPRLLTTNKKPASIKQGTEVPYVLYNRKDEVKNIEFKEAVLGLQVTPHISNDK
KILLDLVVTQNSPNSTGSTLQGLVTIDKQELNTQVFAQHGETIVLGGIFQHLTAKGEDRVPILGSIPVLKKLFSHSSDKI
SKRELVIFVTPYIVKNGQPQISSHSSQKLAPK

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=312069 D1105_RS01045 WP_005614439.1 223857..225155(+) (comE) [Actinobacillus pleuropneumoniae strain 8329]
ATGCGAAAAATGATTTGGTTGCTCCTGTTTATATTAACGCCGGTTATGGCAAATACTTTTTCTCTCTCGCTGAAAAATGC
GCCGACTGCGGAAATTTTAAGTTATTTAGCCGAAGAATACGGTAAAAATATCGTGTTAAGCGACAATATTGAGACAAATA
CTACATTAAGAATTGAAAATAGTGATTTTGACAGCGTATTAAAAAGTATTACTCGGGCGAATAAACTCACGAGTGCATAC
GAAAAACAGATCTATTTTATCGGGCATAAAAAAGATGAGAAAGCGGCTACGGCAATAGGCGTAAATTCTGATTTATTGAA
ACCTAAATTGATCACTAAGACAATAAAATTAGATTATGCGAAGGCGGCGGAAGTGATTGAATCTTTAACTAAAGGAAGCG
GCCATTTTTTATCGGAAAACGGCTATTTGCATTTTGATGATCGTAGTAACAGTTTGATTATTAAAGACAGTCCGGAATCG
ATGAAAAATATCGTGCAATTAATCAGAAACTTAGATAAACCGACCGAACAGATTGCAATTGAAGCCAGAATTGTCACGAT
AAGCAGTGAGAATTTACAAGAATTAGGTGTGCGTTGGGGGATATTTGCTCCTACAAACGGACACCATAAAATTGCCGGTT
CGCTTGAAGCGAACGGATTACCGAATACTAATCGTTTAAACGTAAATTTTCCGGTAAATAATGCCGCTTCTATTGCACTA
CAAGTGGCGAAAATTAATGGGCGAGTGCTTGATTTGGAACTTACCGCTTTGGAACAAGAAAATAATGTTGAGATTATCGC
CAGCCCTCGTTTATTAACCACCAATAAGAAGCCGGCAAGCATTAAGCAAGGAACGGAAGTTCCTTATGTGCTGTATAACC
GTAAAGACGAAGTGAAAAATATCGAATTTAAAGAAGCGGTTTTAGGTTTACAAGTGACGCCGCATATTTCAAACGATAAG
AAAATTTTGCTCGATTTGGTAGTCACCCAAAACTCACCTAATTCGACCGGTTCAACCCTACAAGGCTTAGTGACGATTGA
TAAGCAGGAACTGAATACGCAAGTATTTGCTCAGCATGGCGAAACGATTGTGCTAGGCGGTATTTTTCAGCATTTAACCG
CAAAAGGTGAGGACAGAGTGCCGATTTTAGGTTCGATTCCGGTACTTAAAAAGTTATTTAGCCATTCTAGTGATAAAATT
AGTAAGCGTGAACTAGTGATTTTTGTGACGCCTTACATTGTTAAAAACGGACAACCGCAAATTTCCTCACATTCTTCACA
GAAATTAGCGCCAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(112-177)

Bacterial type II and III secretion system protein (Secretin)

(256-415)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3G2M2X7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Glaesserella parasuis strain SC1401

66.265

96.065

0.637
  comE Haemophilus influenzae Rd KW20

53.037

99.074

0.525
  comE Haemophilus influenzae 86-028NP

52.804

99.074

0.523
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

96.065

0.407
  pilQ Vibrio cholerae strain A1552

42.41

96.065

0.407
  pilQ Vibrio campbellii strain DS40M4

40.326

99.306

0.4

Multiple sequence alignment