Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   D1107_RS04315 Genome accession   NZ_CP031860
Coordinates   968808..969254 (-) Length   148 a.a.
NCBI ID   WP_005597397.1    Uniprot ID   B0BPG5
Organism   Actinobacillus pleuropneumoniae strain 3906     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 963808..974254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D1107_RS04290 (D1107_04330) yacG 964715..964903 (-) 189 WP_237593094.1 DNA gyrase inhibitor YacG -
  D1107_RS04295 (D1107_04335) coaE 964885..965523 (-) 639 WP_005601202.1 dephospho-CoA kinase -
  D1107_RS04300 (D1107_04340) - 965545..966183 (-) 639 WP_005604550.1 prepilin peptidase -
  D1107_RS04305 (D1107_04345) pilC 966210..967412 (-) 1203 WP_237593095.1 type II secretion system F family protein Machinery gene
  D1107_RS04310 (D1107_04350) pilB 967396..968790 (-) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  D1107_RS04315 (D1107_04355) pilA 968808..969254 (-) 447 WP_005597397.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  D1107_RS04320 (D1107_04360) radA 969436..970815 (+) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  D1107_RS04325 (D1107_04365) - 970859..971092 (-) 234 WP_005615190.1 YgjV family protein -
  D1107_RS04330 (D1107_04370) folA 971219..971707 (-) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  D1107_RS04335 (D1107_04375) proB 971794..972885 (+) 1092 WP_005601213.1 glutamate 5-kinase -
  D1107_RS04340 (D1107_04380) - 972898..973674 (+) 777 WP_005597402.1 TSUP family transporter -

Sequence


Protein


Download         Length: 148 a.a.        Molecular weight: 15980.38 Da        Isoelectric Point: 7.6257

>NTDB_id=312024 D1107_RS04315 WP_005597397.1 968808..969254(-) (pilA) [Actinobacillus pleuropneumoniae strain 3906]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFPANFCASN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=312024 D1107_RS04315 WP_005597397.1 968808..969254(-) (pilA) [Actinobacillus pleuropneumoniae strain 3906]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACTATTACAGTAACGGGGAA
AGGGAATCTACAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCCGGCAAATTTCTGCGCATCAAATTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB B0BPG5

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.919

100

0.689

  pilA Haemophilus influenzae 86-028NP

55.102

99.324

0.547

  pilA Haemophilus influenzae Rd KW20

53.061

99.324

0.527


Multiple sequence alignment