Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CH628_RS04465 Genome accession   NZ_CP031681
Coordinates   835731..836423 (+) Length   230 a.a.
NCBI ID   WP_105886719.1    Uniprot ID   -
Organism   Haemophilus influenzae strain P669-6977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 830731..841423
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CH628_RS04445 (CH628_04445) ampD 832002..832562 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CH628_RS04450 (CH628_04450) pilA 832677..833126 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CH628_RS04455 (CH628_04455) pilB 833123..834517 (+) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  CH628_RS04460 (CH628_04460) pilC 834514..835734 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  CH628_RS04465 (CH628_04465) pilD 835731..836423 (+) 693 WP_105886719.1 A24 family peptidase Machinery gene
  CH628_RS04470 (CH628_04470) rho 836477..837739 (-) 1263 WP_015701557.1 transcription termination factor Rho -
  CH628_RS04475 (CH628_04475) metJ 837987..838304 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  CH628_RS04480 (CH628_04480) - 838318..838704 (-) 387 WP_105886720.1 Cu(I)-responsive transcriptional regulator -
  CH628_RS04485 (CH628_04485) - 838781..838987 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  CH628_RS04490 (CH628_04490) - 839073..839279 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -
  CH628_RS04495 (CH628_04495) - 839365..839571 (+) 207 WP_005656255.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26743.73 Da        Isoelectric Point: 9.0977

>NTDB_id=309890 CH628_RS04465 WP_105886719.1 835731..836423(+) (pilD) [Haemophilus influenzae strain P669-6977]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYVELFPQNRSPFQPHFASIQQKKCGHILRYFFSIGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWLAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIHRKKKEFLPFAPFMNLSAIIIYLVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=309890 CH628_RS04465 WP_105886719.1 835731..836423(+) (pilD) [Haemophilus influenzae strain P669-6977]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACACG
TTTACAGCAAGAGATTTATGCGACTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTTAGTATTGGGGTTGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACACCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTACTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGCTTGCAAAATTCTATTATGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAGGAAGAAAAAAGAATTTTTACCTTTTGCCCCTT
TTATGAACTTATCGGCTATCATTATTTATCTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae Rd KW20

97.826

100

0.978
  pilD Haemophilus influenzae 86-028NP

96.957

100

0.97

Multiple sequence alignment